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文献和实验IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
may be used. Try to avoid having more than a monolayer of embryos (tilt vial to increase surface area). Devitillenize with a methanol shock. Remove the aqueous (bottom = fix) phase completely (use a drawn out pipet) as well as one pipetful of heptane
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS (Paul MacDonald) Dechorionate directly on apple juice/agar plates with 50-60% chlorox; embryos will float to the surface after 2-3'. Collect embryos
service, any antibody, from any company, from any where, at any time... e-mail contacts were positive at first and I liked their sheep logo, but they were not interested in supporting ASSIM more and more websites to order on-line and also access
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