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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
, contaminating the sterile media. 1. After sterilization and cooldown, increase the airflow until the vessel pressure reaches ~10 psi. 2. Shut down the air service valve at the copper pipe on the wall of the fermentor room. This activates the exhaust
FREEZING TISSUE FOR CRYOSTAT SECTIONING
All tissue types except muscle: Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc, placing the freshly biopsied
amount of OCT compound onto a cork disc, placing the freshly biopsied tissue onto that and then dropping the disc upside down into the liquid nitrogen. Upon reaching the laboratory the frozen sample is attached to a cryostat chuck . To achieve
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