PP 50ML OPEN TOP TUBE,29X104MM

人单核细胞和中性粒细胞

M199 + 35g NaHCO3 + 25mM Hepes + 5 mM glutamine + 50 ug/ml Gentamycin.1M Hepes: 119.1 g Hepes + 500 ml dH2 O.Media A: 1X HBSS (10X:50ml) + 10 mM Hepes (1M: 5 ml) + 5 mM EDTA (0.5M: 5ml) + 2.5 % FBS (12.5 ml) in 500 ml.Media B: RPMI1640 470 ml + Gent

原创：western protocol

-Cl, pH8.0 1.2114 20% (v/v) Glycerol 20g 4% (w/v) SDS 4g 0.2% Bromophenol blue 0.2g 200mM DTT 20ul 5x Running buffer: 1 L 15.1 g Trizma base (= 125m M) 94g Glycine (= 1.25 M) 50ml 10%SDS (= 0.5%)--add last Do not adjust the pH!! 10x Blotting

Hints and precautions for the care, feeding and breeding of Neurospora

, above 30°C. We routinely make crosses on SC slants in 18 X 150 mm tubes, using foam or cotton plugs. Push-on metal or plastic caps should be avoided with cross tubes because they result in more rapid water loss and are more prone to contamination