SCREW, HEX HEAD SS 4-40 X 1/2(

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

  (1)  Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2)  Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in a screw cap glass vial) for 15 min

Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shea

from the reservoir).     5)      Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head

3% TREVIGEL PROTOCOL for 3 bp resolution

can be obtained by running the gel in 1X TAE at 5 volts/cm for 2 hours with a 40 cm gel. For SSR marker screening in 20 x 40 cm gels: For 400 ml of gel solution: At room temperature, stir 350 ml 1X TAE buffer in a 1 Liter screw cap bottle