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文献和实验【转帖】Top 100 proteomics publications
. Keller et al. (2005) A uniform proteomics MS/MS analysis platform utilizing open XML file formats. Mol Syst Biol 100. Barsnes et al. (2006) MassSorter: a tool for administrating and analyzing data from mass spectrometry experiments on proteins
RNA Purification Protocol for 100-200 mg of Animal Tissue
设备 1. Centrifuge capable of 3,000-5,000 x g 2. High speed centrifuge tube with capacity of at least 15 ml 3. Ice bath 4. Ground pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen
Clonality - X Chromosome Inactivation Assay
(Life Technologies) 10X PCR buffer (100 mM Tris-HCL pH 8.3, 500 mM KCL, 15 mM MgCl2, 0,01 % w/v gelatin, autoclaved) (Perkin Elmer) dNTP, each 10 mM (Perkin Elmer) 7-deaza dGTP, 10 mM (Boehringer Mannheim) DMSO, minimum 99.5%, GC (Sigma
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