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文献和实验DNA Transposons for Modification of Human Primary T Lymphocytes
A, 1994). HIV-1-derived lentiviral vectors have been shown to transduce a variety of slowly dividing or nondividing cells, including unstimulated T lymphocytes (Blood 96:1309–1316, 2000; Gene Ther 7:596–604, 2000; Blood 101:2167–2174, 2002; Hum Gene Ther
Endothelial Cell Systems - Instructions For Use
proliferating formats: T - 25 FLASK 6 - Well Plate 96-Well Plate T - 75 FLASK 12 - Well Plate T - 150 FLASK 24 - Well Plate T - 225 FLASK 48 - Well Plate 1. Check all containers for leakage or breakage
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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