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文献和实验Immunohistochemisty-Fluorescence Protocol
Materials Cytokine-specific Primary Antibodies unlabeled or biotinylated antigen-affinity purified polyclonal antibodies (R&D Systems ''AF'' or ''BAF'' series) or selected monoclonal antibodies. Secondary Antibodies
Immunohistochemistry: Fluorescence Protocol 2.0
Materials Cytokine-specific Primary Antibodies R&D Systems 'AF' or 'BAF' series ) or selected monoclonal antibodies. Secondary Antibodies and Secondary Reagents Biotinylated donkey anti-goat IgG (Jackson
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
may not be necessary for all tissues and shorter fixation times may be preferable as X-gal staining may be decreased by lengthy fixation. Embed tissue in OCT (Miles) or gelatin/sucrose mounting medium and freeze on metal chucks cooled in liquid N2
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