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文献和实验Purification of Plasmid from 50 ml-culture
the supernatant. Remove all of the fluid using pipetman. 10. Dissolve the pellet in 600 ul of TE. Transfer the solution into a microfuge tube. 11. Add 200 ul of 8M LiCl. Mix well, and then spin the solution at 14,000 rpm for 5 min at 4 C. 12
ES CELL DNA EXTRACTION: TUBE ME
for 30 min to precipitate the DNA . (can be stored indefinitely at 0℃ or at -20℃). 15. Centrifuge at 12,000xg for 10 min, 0℃. (Try 4℃, microfuge at max (16,000), 10 min). (Remember to point tube hinges out, to locate pellet even if it's invisible
Shorty DNA Prep (per Adam 08/12/04)【University of Chicago】
%ethanol. 8. Mix by inversion and spin 5minutes at top speed in micro-centrifuge. 9. Pour off liquid and dry pellet by letting it sit upside down on a paper towel for 1hour. 10. Once the tube is dry, add 100µl TE (1X I would not use 10
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