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文献和实验Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy
实验步骤 1. Specimen preparation: Golgi-Cox 1) This protocol closely follows the protocol of Mayerich et al.7 . 2) The C57BL/6J mouse is deeply anesthetized using isoflurane inhalant anesthesia
(e.g. 70% ethanol) between operations and allow a minimum of 15 minutes between handling different cell lines.Wherever possible maintain separate bottles of media for each cell line in cultivation.Examine cultures and media daily for evidence of gross
Subculture of Suspension Cell Lines
separate bottles of media for each cell line in cultivation. Examine cultures and media daily for evidence of gross bacterial or fungal contamination. This includes medium that has been purchased commercially. Quality Control all media and reagents
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