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文献和实验/mL X-Gal (5 -Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) in dimethylformamide store at -20°C in small aliquots. X-gal Mix (make fresh just before use) 10 mL Staining solution 0.25 mL X-gal Stock (final = 1 mg/mL). Be sure
, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods
are described: PCR clamping, fluorescence-based recognition, and microarray platform. The first two methods are performed in solution, while the microarray method uses a solid surface.
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