Use of Peptide Nucleic Acids (PNAs) for Genotyping by Solution and Surface Methods
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Peptide nucleic acids (PNAs) are synthetic oligonucleotide analogues based on a pseudopeptide backbone that bind complementary
DNA or RNA with high affinity and specificity. In this chapter, three PNA-based genotyping assays are described: PCR clamping,
fluorescence-based recognition, and microarray platform. The first two methods are performed in solution, while the microarray
method uses a solid surface.
