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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD204
- 级别：
CD204 Antibody
- 目录编号：
CD204 Antibody
- 克隆性：
单克隆
- 抗原来源：
大鼠
- 保质期：
CD204 Antibody
- 抗体英文名：
Cyanine5.5 anti-mouse CD204 Antibody/PerCP
- 抗体名：
CD204 Antibody
- 标记物：
PerCP
- 宿主：
大鼠
- 适应物种：
小鼠
- 免疫原：
CD204 Antibody
- 亚型：
CD204 Antibody
- 形态：
CD204 Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.2 mg/mL
- 规格：
25 ug/100 ug
| 规格： | 25 ug | 产品价格： | ￥3300.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥7400.0 |
Description ：CD204, also known as scavenger receptor A (SR-A) and the macrophage scavenger receptor(MSR), is 220 kDa, trimeric type II transmembrane protein, with one scavenger receptorcysteine-rich domain (SRCR). It is a phagocytic pattern-recognition receptor (PRR) expressedon macrophages and dendritic cells. CD204 is a receptor mediating recognition andinternalization of low-density lipoprotein (LDL) by macrophages and plays a critical role inatherogenesis. CD204 also recognizes apoptotic cells, modified lipid proteins, and exogenouspathogen-associated molecular patterns (PAMPs), which results in the induction of innateimmune and inflammatory responses. CD204 can act as a co-receptor for Toll-like receptors,such as TLR3, TLR4, or TLR9, to facilitate the expression of proinflammatory cytokines.CD204 has been implicated in several pathological processes such as Alzheimer’s disease,sepsis, ischemic injury, and coronary artery disease.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Rat
Immunogen ：Recombinant mouse CD204 extracellular domain
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation ：The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration ：0.2 mg/mL
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cellsin 100 µL volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
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文献和实验大部分老师做流式实验时，会用到空白对照、单阳对照、同型对照、生物学对照等，用到 FMO 对照的比较少。尽管如此，FMO 对照在设置圈门时仍具有重要的参考价值。 一方面，FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时，可能会出现某些通道信号难以区分的情况，主要是因为其他某个荧光对该通道的干扰很大。FMO 对照（Fluorescence Minus One，荧光扣除一）减少了其中一个通道的荧光抗体，能够帮助衡量其它通道的荧光漏溢情况。 如下图所示，PerCP/Cyanine
免疫亲和层析是以 Fab 片段为基础的无痕亲和细胞分选技术（traceless affinity cell selection, Fab-TACS）。Fab-TACS 运用免疫亲和层析的原理，使用细胞表面抗原分子 CD marker 中的 Fab 片段来进行捕捉及释放目的细胞。以抗体的 Fab 片段取代一般具有高亲和力的完整抗体，实现试剂与细胞的可逆结合，直接从全血或其他血液样本中分选目的细胞。 Fab-TACS 柱子填充了包被有 Strep-Tactin® 多聚体的细胞级琼脂糖基质，偶联
CD11a/CD11b/CD11c 分子 CDlla 常用单克隆抗体或代号：MHM24， 2F12； CRIS―3(LFA―lα链，整合素α1) 主要表达细胞：Leu[A5] 分子质量(kDa)和结构：spl80(整合素α) 功 能：与1CAM-l(CD54)、ICAM-2(CDl02)、ICAM-3(C1350)结合，介导细胞黏附；与JAMl结合，参与白细胞穿过内皮细胞
