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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
CD140b (PDGFRβ)
- 级别：
CD140b (PDGFRβ) Antibody
- 目录编号：
CD140b (PDGFRβ) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
CD140b (PDGFRβ) Antibody
- 抗体英文名：
TotalSeq™-A0129 anti-human CD140b (PDGFRβ) Antibody
- 抗体名：
CD140b (PDGFRβ) Antibody
- 标记物：
CD140b (PDGFRβ) Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
CD140b (PDGFRβ) Antibody
- 亚型：
CD140b (PDGFRβ) Antibody
- 形态：
CD140b (PDGFRβ) Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
10 µg
Description ：CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growthfactor family. The identity of the growth factor bound to the receptor determines whether thefunctional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β.CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with apredicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety ofmesenchymal-derived cells and is preferentially expressed on some tumors such asmedulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cellproliferation. CD140b has been shown to interact with a number of kinases (including Raf-1,NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2,Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3and nexin sorting molecules. CD140b has been implicated in several disease states includingatherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numeroustyrosine residues through both autophosphorylation and ligand-dependent processes.
Verified Reactivity ：Human
Reported Reactivity ：African Green, Baboon, Cynomolgus, Rhesus
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：NIH-3T3 cells transfected with human PDGFRbeta
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验1. Vogel W, et al. 2002. Haematologica 88:126.
2. Arima, S., et al 2011. Development. 138:4763. PubMed.
Product Citations：
1. Hao Y, et al. 2021. Cell. 184:3573. PubMed
（ β－ EP） ，再与HRP 标记的羊抗人抗体结合，形成抗体 - 抗原 - 酶标抗体复合物 ，经过彻底洗涤后 加 底物TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的 人 β－内啡肽 （ β－ EP） 呈正相关。用酶标仪在 450 nm波长下测定吸光度（ OD 值）， 通过标准曲线 计算样品 中 人 β－内啡肽 （ β－ EP） 浓度。 试剂盒组成 ：
（二）CD19 CD19是一种属于Ig超家族成员、分子量为95kDa的穿膜糖蛋白，分布于B细胞表面，其相应的生理性配体尚不清楚。CD19与B细胞活化和信号的转导有关：（1）CD19单克隆抗体可诱导胞浆内多种底物迅速发生磷酸化；（2）CD19胞浆区可被一种丝氨酸激酶催化而发生磷酸化；（3）CD19胞浆区与src激酶家族Lyn稳定的结合。最近提出一个B细胞最佳信号放大的双重抗原结合模型，这个模式认为B淋巴细胞BCR/Igα、Igβ与抗原结合后，使CD19与CD21相互接近形成复合物，外来抗原
密切相关。七十年代，研究人员用凝胶梯度电泳等 生物 化学方法发现了几十种血小板表面膜蛋白，其中一组被称为糖蛋白I（glycoprotein I, GPⅠ）复合物的膜蛋白与血小板“粘附”功能有关。这组复合物又可分为GPⅠb-Ⅸ复合物和GPⅠa-Ⅱa 复合物两类。1979年，笔者赴法进修血液学，有机会通过杂交瘤技术，鉴定出国际上第一个抗血小板膜糖蛋白I的单克隆抗体（AN51）。这株单克隆抗体（以下简称“单抗” ），与正常血小板特异性结合后，血小板就不能通过vW因子“粘附”到破损
