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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
9999
- 靶点：
CD117
- 级别：
anti-human CD117 (c-kit) Antibody
- 目录编号：
anti-human CD117 (c-kit) Antibody
- 克隆性：
单克隆
- 抗原来源：
小鼠
- 保质期：
anti-human CD117 (c-kit) Antibody
- 抗体英文名：
TotalSeq™-A0061 anti-human CD117 (c-kit) Antibody
- 抗体名：
CD117 抗体
- 标记物：
anti-human CD117 (c-kit) Antibody
- 宿主：
小鼠
- 适应物种：
人
- 免疫原：
anti-human CD117 (c-kit) Antibody
- 亚型：
anti-human CD117 (c-kit) Antibody
- 形态：
anti-human CD117 (c-kit) Antibody
- 应用范围：
PG
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
10 ug
TotalSeq™-A0061 抗人 CD117 (c-kit) 抗体详细如下：
Description ：CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cellfactor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferationand survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
Verified Reactivity ：Human
Reported Reactivity ：Cynomolgus, Cow
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：MOLM-1 megakaryocytic cell line
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
Description ：CD117 is a 145 kD protein tyrosine kinase also known as c-Kit. It is a receptor for stem cellfactor or c-Kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells(approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML).CD117 binding of c-Kit ligand induces phosphorylation of CD117 and stimulates proliferationand survival of primitive hematopoietic stem cells as well as erythroid-committed and granulomonocytic committed cells.
Verified Reactivity ：Human
Reported Reactivity ：Cynomolgus, Cow
Antibody Type ：Monoclonal
Host Species ：Mouse
Immunogen ：MOLM-1 megakaryocytic cell line
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation ：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer underoptimal conditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application ：PG - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies arecompatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.
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文献和实验该产品被引用文献
Application References(PubMed link indicatesBioLegend citation)：
1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations：
1. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
2. Hao Y, et al. 2021. Cell. 184:3573. PubMed
1. Broudy VC, et al. 1999. Blood 94:1979. (IF, IP)
2. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
3. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
4. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations：
1. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
2. Hao Y, et al. 2021. Cell. 184:3573. PubMed
文献支持
A0061 抗人 CD117 (c-kit) 抗体
￥6500
