Description :CD140b is a cell surface tyrosine kinase receptor for members of the platelet-derived growthfactor family. The identity of the growth factor bound to the receptor determines whether thefunctional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β.CD140b contains two immunoglobulin-like domains and a tyrosine kinase domain with apredicted molecular weight approximately 124 kD. CD140b is widely expressed on a variety ofmesenchymal-derived cells and is preferentially expressed on some tumors such asmedulloblastoma. Binding of B-chain containing PDGF molecules can stimulate cellproliferation. CD140b has been shown to interact with a number of kinases (including Raf-1,NCK1, FAK, Fyn, others) as well as adaptor molecules and signaling intermediates (Crk, Grb2,Grb4, RasGAP, SHP2, SHC1, others), and has also been shown to associate with integrin β3and nexin sorting molecules. CD140b has been implicated in several disease states includingatherogenesis and oncogenesis. The PDGFRβ is heavily phosphorylated on numeroustyrosine residues through both autophosphorylation and ligand-dependent processes.
Immunogen :NIH-3T3 cells transfected with human PDGFRbeta
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA
Preparation :The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer underoptimal conditions.
Concentration :0.5 mg/mL
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application :PG - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies arecompatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for eachapplication, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2- 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to thecell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent fortitration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™protocol for specific staining instructions.