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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
齐源
- 库存：
999
- 靶点：
Ly-49C/F/I/H
- 级别：
Ly-49C/F/I/H Antibody
- 目录编号：
Ly-49C/F/I/H Antibody
- 克隆性：
单克隆
- 抗原来源：
仓鼠
- 保质期：
Ly-49C/F/I/H Antibody
- 抗体英文名：
anti-mouse Ly-49C/F/I/H Antibody-FITC
- 抗体名：
Ly-49C/F/I/H Antibody
- 标记物：
FITC
- 宿主：
仓鼠
- 适应物种：
小鼠
- 免疫原：
Ly-49C/F/I/H Antibody
- 亚型：
Ly-49C/F/I/H Antibody
- 形态：
Ly-49C/F/I/H Antibody
- 应用范围：
FC
- 保存条件：
2-8℃
- 浓度：
0.5 mg/ml
- 规格：
50 µg
Description ：The 14B11 antibody reacts with a common epitope of Ly-49C/F/I/H family members also knownas Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with otherLy-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linkedhomodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H isexpressed on a subset of NK cells, NK-T cells and some CD8 cells. Ly-49C/F/I, like othermembers of this family, inhibits NK cell activation through the recognition and interaction ofcertain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain anITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1rgene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c miceare reported to lack the Ly-49H and Ly-49I receptors. The 14B11 antibody, when bound to NKcells, is capable of inducing redirected cell lysis and blocking NK cell effector function.
Verified Reactivity ：Mouse
Antibody Type ：Monoclonal
Host Species ：Syrian Hamster
Immunogen ：IL-2-activated killer cells (LAK) from C57BL/6 mice
Formulation ：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation ：The antibody was purified by affinity chromatography, and conjugated with FITC under optimalconditions.
Concentration ：0.5 mg/ml
Storage & Handling ：The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application ：FC - Quality tested
Recommended Usage ：Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
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文献和实验1. Corral L, et al. 1999. Hybridoma 18:359.
2. Hanke T, et al. 1999. Immunity 11:67.
Product Citations：
1. Cao D, et al. 2009. Transpl Immunol. 20:243. PubMed
当FITC在碱性溶液中与抗体蛋白反应时，主要是蛋白质上赖氨酸的r氨基与荧光素的硫碳胺键(thiocarbmide)结合，形成FITC-蛋白质结合物，即荧光抗体或荧光结合物。一个IgG分子中有86个赖氨酸残基，一般最多能结合15～20个，一个IgG分子可结合2～8个分子的FITC，其反应式如下FITC-N=C=S + N-H2-蛋白质 → FITC-NS-C-N-H2-蛋白质常用Marsshall(1958)法标记荧光抗体，也可以根据条件采用Chadwick等标记法或Clark
ml三蒸水中即成； 方法与步骤： 根据Marshall氏法高效价的抗人球蛋白兔免疫血清，分离球蛋白。 1. 用0.15 mol/L NaCl的盐水及0.15 mol/L pH9.0的NaHCO3-Na2CO3缓冲液稀释使每毫升内含抗体10mg，缓冲液为总量的10%； 2. 将以上溶液降温至4℃，按蛋白：荧光素=50—80mg：1mg的比例加入异硫氰酸荧光素，在0—4℃下电磁搅拌12—14h； 3.用半饱和硫酸铵将标记球蛋白
试剂： 抗体球蛋白溶液、异硫氰酸荧光素、3%重碳酸钠水溶液、pH8.0的0.01mol/L PBS、1%硫柳汞。 材料： 离心机及离心管、三角烧瓶（25ml）、冰槽、无菌吸管及毛细滴管、透析袋、玻璃棒、棉线及烧杯（500ml）等。 方法与步骤： 1. 抗体的准备。用0—4℃ pH8.0的PBS将免疫球蛋白溶液浓度稀释为30—40mg/ml，置于三角
