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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
CD42d
- 级别:
CD42d Antibody
- 目录编号:
CD42d Antibody
- 克隆性:
单克隆
- 抗原来源:
仓鼠
- 保质期:
CD42d Antibody
- 抗体英文名:
Cyanine5.5 anti-mouse/rat CD42d Antibody/PerCP
- 抗体名:
CD42d Antibody
- 标记物:
PerCP
- 宿主:
仓鼠
- 适应物种:
小鼠、大鼠
- 免疫原:
CD42d Antibody
- 亚型:
CD42d Antibody
- 形态:
CD42d Antibody
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/ml
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥2300.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5900.0 |
Description :CD42d is an 83 kD surface glycoprotein that non-covalently associates with GPIb and GPIX toform a receptor complex for von Willebrand factor on megakaryocytes and resting platelets.Binding sites for von Willebrand factor and thrombin have been localized to the GPIba chain ofthe GPI-b-V-IX complex. Platelet activation with thrombin cleaves the GPI-b-V-IX complex torelease a 69 kD soluble fragment. Presence of the GPI-b-V-IX complex is important in BernardSoulier syndrome, a rare bleeding disorder.
Verified Reactivity :Mouse, Rat
Antibody Type :Monoclonal
Host Species :Armenian Hamster
Immunogen :Mouse platelets
Formulation :Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation :The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5under optimal conditions.
Concentration :0.2 mg/ml
Storage & Handling :The antibody solution should be stored undiluted between 2°C and 8°C, and protected fromprolonged exposure to light. Do not freeze.
Application :FC - Quality tested
Recommended Usage :Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cellsin 100 µl volume. It is recommended that the reagent be titrated for optimal performance for eachapplication.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
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文献和实验1. Takada K, et al. 1995. Hybridoma. 14:361. (IP)
2. Saito M, et al. 1996. Stem Cells. 14:124. (FC)
3. Sato N, et al. 2000. Hybridoma. 19:455. (IP)
大部分老师做流式实验时,会用到空白对照、单阳对照、同型对照、生物学对照等,用到 FMO 对照的比较少。尽管如此,FMO 对照在设置圈门时仍具有重要的参考价值。 一方面,FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时,可能会出现某些通道信号难以区分的情况,主要是因为其他某个荧光对该通道的干扰很大。FMO 对照(Fluorescence Minus One,荧光扣除一)减少了其中一个通道的荧光抗体,能够帮助衡量其它通道的荧光漏溢情况。 如下图所示,PerCP/Cyanine
(3)同上。两个磷酸化位点都要做,但是可能在不同的病理条件下,侧重点有所不同,这主要体现在你的论文的讨论中。因此建议实验前多做论文研究,查阅一下别人的发表文章,看看与你的病理模型更相关的是哪个位点。 真爱满行囊 非常感谢你 的回复,现在我遇到的问题是我做出来蛋白总量还有216位点是有变化的,9位因为买的是santa curz的抗体,现在死活做不出来,很头痛,我要说明的问题是他的活性降低,我也看到216位点磷酸化水平降低了,可是现在没有第九位的结果(就是没有办法得到第九
间接法免疫荧光组织化学染色步骤 1.检测抗原法以单层细胞培养标本为例介绍此法的染色步骤: (1)取出细胞贴附生长的小盖片,用预热的PBS冲洗2次; (2)4%多聚甲醛(或冷丙酮)固定5~10分钟,晾干; (3)0.01MPBS(pH 7.4)漂洗3次,每次5分钟。 (4)0.3%Triton X-100孵育,室温20分钟,PBS漂洗2次(细胞膜抗原可省略此步骤); (5)非免疫血清(或15%小牛血清)孵育,室温10分钟; (6)滴加特异性一抗(如小鼠抗大鼠










