- ￥1985
- neobioscience
- EHC002b.96
- 深圳
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
欣博盛生物
- 库存：
大量现货
- 应用：
ELISA
- 检测方法：
双抗体夹心法
- 规格：
96T
QuantiCyto® Human IL-1β ELISA kit（人白细胞介素-1β）
品牌： NeoBioscience
产品货号： EHC002b.96
规格： 96T
产品详情
产品名称:QuantiCyto® Human IL-1β ELISA kit（人白细胞介素-1β）
保存温度:4°C，标准品保存于-20°C，请参考说明书
运输温度:Gel Ice
产地:深圳
生产商:欣博盛
灵敏度:3.9pg/ml
检测范围:7.8-500pg/ml
检测方法:双抗体夹心法
检测原理:
欣博盛 QuantiCyto® ELISA试剂盒采用双抗体夹心法：抗人IL-1β单抗包被于酶标板上，标本和标准品中的人IL-1β会与单抗结合，游离的成分被洗去。加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合；抗人IL-1β抗体与结合在单抗上的人IL-1β结合而形成免疫复合物，游离的成分被洗去。加入显色底物，若反应孔中有人IL-1β，辣根过氧化物酶会使无色的显色剂现蓝色，加终止液变黄。在450 nm处测OD值，人IL-1β浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人IL-1β的浓度。
品牌： NeoBioscience
产品货号： EHC002b.96
规格： 96T
产品详情
产品名称:QuantiCyto® Human IL-1β ELISA kit（人白细胞介素-1β）
保存温度:4°C，标准品保存于-20°C，请参考说明书
运输温度:Gel Ice
产地:深圳
生产商:欣博盛
灵敏度:3.9pg/ml
检测范围:7.8-500pg/ml
检测方法:双抗体夹心法
检测原理:
欣博盛 QuantiCyto® ELISA试剂盒采用双抗体夹心法：抗人IL-1β单抗包被于酶标板上，标本和标准品中的人IL-1β会与单抗结合，游离的成分被洗去。加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，生物素与亲和素特异性结合；抗人IL-1β抗体与结合在单抗上的人IL-1β结合而形成免疫复合物，游离的成分被洗去。加入显色底物，若反应孔中有人IL-1β，辣根过氧化物酶会使无色的显色剂现蓝色，加终止液变黄。在450 nm处测OD值，人IL-1β浓度与OD450值之间呈正比，可通过绘制标准曲线求出标本中人IL-1β的浓度。
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文献和实验该产品被引用文献
部分文献引用：
Xu H, Y. J., Gao W, et al. (2014). Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature.
Yue Zhao, J. Y., Shao F. (2011). The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature.
He W, W. H., Hu L, et al. (2015). Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. Cell Research.
Zhang T, Fang Z, Linghu K G, et al. (2020). Small molecule-driven SIRT3-autophagy mediated NLRP3 inflammasome inhibition ameliorates inflammatory crosstalk between macrophages and adipocytes. British Journal of Pharmacology.
Gong YN, Shao F, et al. (2010). Chemical probing reveals insights into the signaling mechanism of inflammasome activation. Cell Research.
Li J, Huang S, Zeng L, et al. (2020). Necroptosis in head and neck squamous cell carcinoma: characterization of clinicopathological relevance and in vitro cell model. Cell Death & Disease.
Li H, Jiang W, Ye S, et al. (2020). P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death & Disease.
Yang Q, L. S., Liu X, et al. (2019). Role of charge reversal in the hemo/immunocompatibility of polycationic gene delivery systems. Acta Biomaterialia.
Yu J, L. S., Qi J, et al. (2019). Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells. Cell death & disease.
Wang S, Ma J, Guo S, et al. (2020). Transcriptomic analysis revealed that multi-walled carbon nanotubes diameter-dependently induced pyroptosis in THP-1 macrophages. NanoImpact.
Ye H, Zhou Y, Ma P, et al. (2020). Analysis of the anti-inflammatory effect of the
aptamer LA27 and its binding mechanism. International Journal of Biological Macromolecules
Xu H, Y. J., Gao W, et al. (2014). Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature.
Yue Zhao, J. Y., Shao F. (2011). The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature.
He W, W. H., Hu L, et al. (2015). Gasdermin D is an executor of pyroptosis and required for interleukin-1β secretion. Cell Research.
Zhang T, Fang Z, Linghu K G, et al. (2020). Small molecule-driven SIRT3-autophagy mediated NLRP3 inflammasome inhibition ameliorates inflammatory crosstalk between macrophages and adipocytes. British Journal of Pharmacology.
Gong YN, Shao F, et al. (2010). Chemical probing reveals insights into the signaling mechanism of inflammasome activation. Cell Research.
Li J, Huang S, Zeng L, et al. (2020). Necroptosis in head and neck squamous cell carcinoma: characterization of clinicopathological relevance and in vitro cell model. Cell Death & Disease.
Li H, Jiang W, Ye S, et al. (2020). P2Y 14 receptor has a critical role in acute gouty arthritis by regulating pyroptosis of macrophages. Cell Death & Disease.
Yang Q, L. S., Liu X, et al. (2019). Role of charge reversal in the hemo/immunocompatibility of polycationic gene delivery systems. Acta Biomaterialia.
Yu J, L. S., Qi J, et al. (2019). Cleavage of GSDME by caspase-3 determines lobaplatin-induced pyroptosis in colon cancer cells. Cell death & disease.
Wang S, Ma J, Guo S, et al. (2020). Transcriptomic analysis revealed that multi-walled carbon nanotubes diameter-dependently induced pyroptosis in THP-1 macrophages. NanoImpact.
Ye H, Zhou Y, Ma P, et al. (2020). Analysis of the anti-inflammatory effect of the
aptamer LA27 and its binding mechanism. International Journal of Biological Macromolecules
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QuantiCyto® Human IL-1β ELISA kit（人白细胞介素-1β ELISA试剂盒）
￥1985
