- ￥500
- BioToolomics
- 英国
- 330201-5ML
- 2025年07月14日
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- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
NHS活化磁性琼脂糖层析填料
产品名称：NHS活化磁性琼脂糖层析填料英文名称：NHS-activated SepFast MAG 4HF
Product Introduction:
NHS (N-hydroxysuccinimide)-activated SepFast MAG is used to make magnetic adsorbents coupled with molecules containing amine groups. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
NHS-activated SepFast MAG media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides.
This pre-activated resin can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
Product Properties:
NHS activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It shows high mechanical rigidity and allows harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with Nhydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFasm MAG media is supplied as a suspension in 100% isopropanol.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
后，将EP管置于磁性分离架上，去除上清，加入封闭液，此时，磁珠的终浓度为5mg/mL，同样置于37℃环境旋转混匀反应1h。 2.5. 封闭完成后，重复2.2步骤，最后将磁珠用保存液保存，此时磁珠的浓度可自定。 三、羧基磁性微球偶联抗体 1. 偶联条件 1.1. 活化剂：EDC和NHS 1.2. 反应温度：37℃环境 1.3. 反应时间： 1.3.1.活化时间：30min 1.3.2.偶联时间：2h
性好、孔径均匀适当、宜于活化等优点，因此得到了广泛的应用。琼脂糖凝胶微球的商品名为Sepharose，含糖浓度为2%、4%、6%时分别称为2B、4B、6B。因为Sepharose 4B的结构比6B疏松，而吸附容量比2B大，所以4B应用最广。基质的活化基质的活化是指通过对基质进行一定的化学处理，使基质表面上的一些化学基团转变为易于和特定配体结合的活性基团。配体和基质的偶联，通常首先要进行基质的活化。多糖基的活化多糖基质尤其是琼脂糖是一种常用的基质。琼脂糖通常含有大量的羟基，通过一定的处理可以引入各种适宜
粒子的表面(或孔内)即可制备亲和吸附介质，该固体粒子通常称为配基的载体。因此，亲和吸附介质又称亲和载体。作为载体的固体粒子应满足下列要求:(1)具有亲水性多孔结构，无非特异性吸附，比表面积大;(2)物理和化学稳定性高，有较高的机械强度，使用寿命长;(3)含有可活化的反应基团，用于亲和配基的固定化;(4)粒径均一的球形粒子。常用的亲和层析载体有:琼脂糖凝胶和交联琼脂糖凝胶、聚丙烯酰胺凝胶、葡聚糖凝胶、聚丙烯酰胺—琼脂糖凝胶、纤维素、多孔玻璃等。其它用于载体的物质还有:聚苯乙烯、淀粉珠、壳聚糖、硅胶
