- ￥500
- BioToolomics
- 英国
- 340204-5ML
- 2025年07月14日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
乙醛活化磁性琼脂糖凝胶6HF
产品名称：乙醛活化磁性琼脂糖凝胶6HF英文名称：Aldehyde-activated SepFast MAG
Product Introduction:
Aldehyde-activated SepFast MAG is a magnetic resin for the preparation of custom magnetic affinity chromatography media. The magnetic particles can be readily controlled and isolated in biological feedstock with the aid of a magnet. Coupling biospecific ligands to aldehyde-activated agarose is a successful and well-documented technique.
Aldehyde-activated SepFast MAG forms chemically stable secondary amine linkages with ligands containing primary amine groups through reductive amination reactions. This pre-activated agarose base matrix can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
There is a choice of 2 different base matrices that suit various molecules to be coupled or purified.
-
Aldehyde-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. Its large pore size is suitable for coupling or purifying large molecules. It shows high mechanical rigidity allowing harsher operational conditions.
-
Aldehyde-activated SepFast 6HF is made of highly cross-linked 6% agarose containing magnetic material. Its moderate pore size is suitable for coupling smaller molecules. It shows high mechanical rigidity allowing harsher operational conditions.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated through oxidation to generate aldehyde groups. It reacts directly with the primary amine groups in molecules to be immobilized in the presence of reducing agents. Aldehyde-activated SepFast MAG media is supplied as an aqueous suspension in 20% ethanol.
Product Series:
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
粒子的表面(或孔内)即可制备亲和吸附介质，该固体粒子通常称为配基的载体。因此，亲和吸附介质又称亲和载体。作为载体的固体粒子应满足下列要求:(1)具有亲水性多孔结构，无非特异性吸附，比表面积大;(2)物理和化学稳定性高，有较高的机械强度，使用寿命长;(3)含有可活化的反应基团，用于亲和配基的固定化;(4)粒径均一的球形粒子。常用的亲和层析载体有:琼脂糖凝胶和交联琼脂糖凝胶、聚丙烯酰胺凝胶、葡聚糖凝胶、聚丙烯酰胺—琼脂糖凝胶、纤维素、多孔玻璃等。其它用于载体的物质还有:聚苯乙烯、淀粉珠、壳聚糖、硅胶
不能发生）,以核小体为单位剪切DNA（电泳显示为DNA ladder）,Prelytic DNA 断裂,线粒体释放多种因子至胞浆中（细胞色素c、AIF）,Caspases级联活化,膜对称性改变（PS外翻） 生理学特征--只影响单个细胞 ,由生理学刺激诱导（如生长因子缺乏、激素环境改变等）,被临近细胞和巨噬细胞吞噬 无炎症反应 第四节 细胞计数及活力测定 一、原理 培养的细胞在一般条件下要求有一定的密度才能生长良好，所以要进行细胞计数。计数结果以每毫升细胞数表示。细胞计数的原理和方法与血
