- ￥500
- BioToolomics
- 英国
- 330101-50ML
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 供应商：
北京百奥创新科技有限公司
- 规格：
5mL;50mL;1L
NHS活化琼脂糖介质4HF
产品名称：NHS活化琼脂糖介质4HF
英文名称：NHS-activated SepFast 4HF
Product Introduction:
NHS (N-hydroxysuccinimide)-activated agarose has a well-proven track record for the preparation and use of custom affinity chromatography media. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
NHS-activated SepFast media forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides. This pre-activated agarose base matrix can be readily employed to make various custom affinity chromatography adsorbents for both small scale and large scale purification applications.
Product Properties:
NHS activated SepFast 4HF is made of highly cross-linked 4% beaded agarose. It shows high mechanical rigidity allowing high flow throughput with reduced back pressure in a packed column.
Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to various molecules.
The base matrix is activated through a long hydrophilic spacer arm (11 atoms) with N-hydroxysuccinimide at the end. It reacts directly with the primary amine groups in molecules to be immobilized to form stable amide bonds. NHS-activated SepFast media is supplied as a suspension in 100% isopropanol.
Product Series:
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文献和实验S.L. Lim, H.W. Ng, M.A. Akwiditya, C.W. Ooi, E.S. Chan, K.L. Ho, W.S. Tan, G.K. Chua, B.T. Tey, Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography, Process Biochemistry, 2018, 69:208-215.
作用。已有报道，在可见光（需加光感剂如亚甲基蓝）和紫外光（无需光感剂）条件下核酸可与蛋白质直接交联。反应膜 活化膜的制造大受关注。所谓活化膜是指表面可提供直接反应的化学基团的膜介质。无需其它试剂，液体样本可直接结合到活化膜表面，这样体现它的好多优势，特别是应用到微量分析中。用来活化膜介质的化学试剂有多种，具有代表性是类似于叠氮化合物一些物质，它们具有一般的反应共性，然而也存在相似的弊端。反应膜存在几个问题：第一个问题是失活。随时间的延长，经化学活化的反应膜会因为表面和空气发生反应而失活。膜活化所用
的浓度降低点，这也是个办法。 2)请问有没有合成过配体为FMN的亲和胶? 亲和的填料合成过20来种，你是希望用它来纯化某种脱氢酶吗，我看FMN可偶联的有限，倒是FAD有活泼的氨基好偶联，何况它们几乎是同一个辅酶，你是不是考虑把FAD连上去。当然FMN的结构上也有个仲胺，可以偶联到带长手臂的环氧活化的填料上，而溴化氰活化或别的活化的介质都不大好，因此我觉得这个没有什么问题。 此外如果是以FMN为辅酶的，那我还可以建议你试试蓝色琼脂糖凝胶，因为它的配基的结构和FMN
9.0 NaHCO3抽洗。4.偶联蛋白20ml 4B液体相当于一半的固相载体。事先将需偶联的抗原（或抗体）蛋白200mg置于0.1Mol/L pH 9.0 NaHCO3液中透析数小时(一般偶联量为10~30mg/g载体)。5.将活化的琼脂糖迅速倒入蛋白液中（在1.5min内，从抽洗到偶联），4℃缓慢搅拌过夜，使蛋白与活化的琼脂结合。6.在烧结玻璃漏斗中用200ml偶联缓冲液（0.1mol/L碳酸氢钠，0.5mol/L氯化钠，pH8.3）洗偶联介质一次。7.转移偶联介质至含100ml阻断缓冲液（1mol/L乙醇
