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Creative Biogene offers cell line development services to accommodate a variety of stages of the drug development process as follows:
• Cloning of the provided cDNA or your gene of interest into a suitable expression vector. Codon
optimizations can be introduced for the high yield production.
• Transfection of host cell line which is compliance with FDA guideline.
• Incubation and cell line recovery/growth.
• Colony isolation, clone screening using ELISA, Western, or cell based assay of your interest, such as
intracellular calcium, IP1, intracellular cAMP, thallium flux or membrane potential assay, et al.
• Stable cell line expansion.
• Cell line and supernatant harvest & processing.
• Characterization and assay according to your requirements (optional).
• Free cell line backup service for two years from delivery of cell clones.
Creative Biogene "Lenti-Stable" platform engineers in house lentiviral vector for high efficient gene integration into cell genome with extremely low false positive clones. Creative Biogene offers cell line development services to accommodate a variety of stages of the drug development process as follows:
Construct your gene into our lentiviral vector with desired selection marker.
• Generate the lentivirus particles.
• Sequentially transduce the cell line of your interest.
• Select the clones constantly expressing your gene at high level.
• Validation the genomic integration of gene via genomic PCR.
• Validation the high-expression clone by Western Blot (if applicable).
• Deliverable: Two cryogenic preserved vials of stable cells and final report.
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文献和实验Generation of a Stable Cell Line for Constitutive miRNA Expression
Here, a protocol is described to generate stable cell lines for constitutive expression of single cellular or viral miRNA precursors in absence of infection. The procedure comprises cloning of the precursor sequence, generation of the lentiviral expression vector
Generation of Stable Cell Lines Expressing Chemokine Receptors
receptors is also becoming increasingly important in high throughput screening approaches for the identification of chemokine receptor antagonists and agonists. The basic steps in the generation of stable cell lines expressing chemokine receptors
Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
, and telomerase by retrovirally mediated transduction and selection for neomycin resistance are described. However, stable integration of an immortalizing gene stabilizes proliferative capacity, but not the differentiated chondrocyte phenotype. Thus, strategies
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