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Creative Biogene has built-up leading scientific, technical and commercial track records, successfully advancing therapeutic programs for numerous major industry and academic partners in a wide range of disease areas. With roots as a pioneer of genome-scale RNAi screening and long-standing contributions towards refining best practices in this field, Creative Biogene provides leading functional genomics technologies including RNA interference (RNAi) tools for gene silencing (siRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. All RNAi research projects carried out by Creative Biogene are custom-designed to address each client's specific experimental, strategic and budgetary guidelines, while always meeting the highest scientific standards in the field. Creative Biogene has developed a unique expertise of handling all steps involved from dsRNA design and production to fully automated screening and data analysis. RNA Interference (RNAi) is a powerful tool used to effectively regulate gene expression and is critical in the drug discovery process. Knockdown of target genes with RNAi reagents can be used to facilitate the identification and validation of drug targets and to elucidate biochemical and cell signaling pathways. RNAi is a specific, potent, and highly successful approach for loss-of-function studies in virtually all eukaryotic organisms. RNAi also offers the promise of a new, faster, more cost-effective, and more versatile alternative to current transgenic mouse knockout technologies.
Creative Biogene utilizes many different RNAi technologies to achieve robust knockdown and help you understand complex biological systems. The professional technical services of Creative Biogene's RNAi service team can help clients achieve the maximal value of RNAi technology and avoid some experimental errors. Creative Biogene's research team will work with you to develop much more extensive and effective solutions. Creative Biogene has been granted multiple patents on DNA-based, shRNA and miRNA platforms that are popularly used as gene silencing tools. Compared to other shRNA-expressing and miRNA-expressing plasmids, Creative Biogene's platform design allows high-level expression of shRNA and miRNA. Viruses that can integrate into the chromosomes of most host cells are often used to carry shRNA or miRNA for RNAi screening because of their high transduction rates.
Creative Biogene offers custom RNAi services for your scientific research as follows:
• siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or
GeneBank ID and so on.
• Choice of shRNA and miRNA vector.
• siRNA, shRNA and miRNA transfection optimization service.
• Test of inhibitory effect: the mRNA expression is detected by RT- PCR; the protein expression is
detected by Western Blot.
• Provision of experimental reports RNAi sequence, RT-PCR test report, Western Blot picture and so
on.
• Custom preparation of shRNA and miRNA expressing lentiviral particles, determination of the virus
titer and establishment of stable cell lines.
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文献和实验利用BLOCK-iT8482; RNAi技术，进行简单、高效的RNAi分析！
选择BLOCK-iT™ Dicer RNAi 试剂 盒，利用RNA干扰技术（RNA interference，RNAi）进行简单、快速的基因阻断，您不必再花费时间设计siRNA oligo序列及检测它们的效率，就可直接获得RANi结果。 功能基因阻断研究的简单、快速的方法 RNA干扰（RNAi）正在成为一种最受欢迎的技术之一，用于在功能分析试验中有效地阻断特异基因的表达。通过这种方法，利用长度为21-23个核苷酸的小干扰RNA
Microwave Processing Techniques for Biological Samples in a Service Laboratory
The author’s interest in microwave (MW) processing of biological samples for electron microscopy was initially stimulated by presentations and papers by Dr. Gary Login (Login and Dvorak, 1988 , Login and Dvorak, 1993 , Login and Dvorak, 1994
利用RNAi的威力RNA干扰（RNA interference RNAi）正在迅速成为最受欢迎的阻断特异基因表达的技术。RNAi技术允许科学家关闭他们想要研究的基因，这是一种全新的令人激动的研究方法，通过研究基因表达缺失时细胞的表型，以确定基因的功能。最初，通过在无脊椎动物如：果蝇（Drosophila）和线虫（C. elegans）导入长的双链RNA，揭示了RNAi是一种降低特异基因表达的强有力的方法（综述1，2）。现在可以使用修饰的RNAi阻断技术，在真核细胞中进行分析。BLOCK
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