- 询价
- Cell Signaling Technology
- 2368
- USA
- 2025年07月13日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）
- 不限
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- 详细信息
- 技术资料
- 适应物种：
不限
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 抗体英文名：
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)
- 是否单克隆：
0
- 保存条件：
-20°c
- 库存：
大量
- 供应商：
CST
- 抗原：
/
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368
| Applications | Reactivity | Sensitivity | Source |
|---|---|---|---|
| W IP IHC-P IF-IC | All | Transfected Only | Rabbit |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
Specificity / Sensitivity
DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies) fused to either the amino- or carboxy-terminus of targeted proteins.The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from COS or 293T cells, mock transfected (lane 1) or expressing DYKDDDDK-tagged p73 (lane2), DYKDDDDK-tagged FKHRL1 (lane 3) or truncated DYKDDDDK-tagged huntingtin (lane 4), using DYKDDDDK Tag Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded COS cells, control (left) or DYKDDDDK-FKHRL1 transfected (right), using DYKDDDDK Tag Antibody.
IF-IC
Confocal immunofluorescent analysis of COS cells, untransfected (right) or transfected with a construct overexpressing DYKDDDDK-tagged FKHRL1 (left) using DYKDDDDK Tag Antibody.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).
Application References
- Pfeffer, L.A. et al. (2009) Mol Biol Cell 20, 3810-7. Applications: IF-IC (In Cells)
- Forrester, M.T. et al. (2011) Biotechniques 50, 41-5. Applications: IF-IC (In Cells)
- Zhao, J.X. et al. (2011) J Biol Chem 286, 16426-34. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 8146 DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)
Anti-FLAG® is a registered trademark of Sigma-Aldrich.
For Research Use Only. Not For Use In Diagnostic Procedures.
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