- 询价
- Cell Signaling Technology
- 4138
- USA
- 2025年11月27日
- W, IF-IC
- Rabbit
- H,M,R,Hm,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cyclin B1 Antibody
- 抗原：
syntheic peptide corresponding to residues near the amino terminus of human cyclin B1
- 应用范围：
W, IF-IC
- 宿主：
Rabbit
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Hm,Mk
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
2
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Hm Mk | Endogenous | 58 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cyclin B1 Antibody detects endogenous levels of total cyclin B1. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a syntheic peptide corresponding to residues near the amino terminus of human cyclin B1. Antibodies are purified using peptide affinity chromatography. Western Blotting
Western blot analysis of extracts from various cell types using Cyclin B1 Antibody. IF-IC
Confocal immunofluorescent analysis of HeLa cells using Cyclin B1 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red) showing concentrated cyclin B1 staining surrounding mitotic chromatin. |
| Background | Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin B1 binding, phosphorylation of cdc2 at Thr161 and dephosphorylation of cdc2 at Thr14/Tyr15 (1-5). The protein levels of CDK inhibitors and the CDK-associated cyclins are regulated by phosphorylation, ubiquitination and degradation, allowing for a stoichiometric regulation of cell cycle events (6). Four cyclin B1 phosphorylation sites (Ser126, 128, 133 and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint (8-10). Phosphorylation of cyclin B1 is required for cdc25C-dependent dephosphorylation of Tyr15 within cdc2 and subsequent cdc2/cyclin B1 activation (11). While cdc2/cyclin B1 itself can phosphorylate Ser126 and Ser128 (8), polo-like kinase 1 phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 as well (11-13).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验of microinjecting cRNA to proteins of interest that have been coupled to a fluorescent protein using cyclin B1 as an example.
2 瓶 底物工作液（ TMB Solution ） 12ml 第一抗体工作液（ Biotinylated Antibody ） 12ml 终止液 （ Stop Solution ） 12ml
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
that has been kept -20°C. Incubate the cells for at least two hours. Wash once with staining buffer and decant. Add the primary anti-cyclin antibody in a volume of 200 µ l. Incubate overnight at 4°C. Wash twice with cold cyclin staining buffer and add the Cy
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