- 询价
- Cell Signaling Technology
- 3656
- USA
- 2025年09月16日
- 免疫荧光（IF）
- 小鼠
- 人,小鼠,大鼠
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
GFAP (GA5) Mouse mAb (Alexa Fluor 555 Conjugate)
- 是否单克隆：
1
- 保存条件：
-20°c
- 级别：
详见MSDS文件
- 适应物种：
人,小鼠,大鼠
- 应用范围：
免疫荧光（IF）
- 宿主：
小鼠
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 抗原：
/
- 库存：
大量
- 规格：
100 ul (100 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (100 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (100 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (100 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Neuroscience
GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656
PhosphoSitePlus ® protein, site, and accession data: GFAP
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-F | H M R | Endogenous | Mouse IgG1 |
Applications Key: IF-F=Immunofluorescence (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3656:
- Immunofluorescence
Specificity / Sensitivity
GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of total GFAP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with native GFAP purified from pig spinal cord. The antibody was conjugated to Alexa Fluor® 555 under optimal conditions with an F/P ratio of 2-6.
IF-F
Confocal immunofluorescent analysis of normal rat cerebellum using GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb #2837 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for direct immunofluorescence of rat cerebellum. The unconjugated antibody #3670 reacts with human, mouse and rat GFAP protein. CST expects that GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) will also recognize GFAP in these species.
Background
The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral, and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin, and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin-forming Schwann cells in the peripheral nervous system (3).
- Eng, L.F. et al. (2000) Neurochem. Res. 25, 1439-51.
- Goebel, H.H. et al. (1987) Acta. Histochem. Suppl. 34, 81-93.
- Jessen, K.R. et al. (1990) Development 109, 91-103.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验：Catalog NumberProduct NameProduct TypeSizeApplication4408SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F HCA4409SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary
神经元维持添加剂 神经元分化添加剂 牛纤维连接蛋白 100X 山羊抗大鼠巢蛋白抗原亲和纯化多克隆抗体 绵羊抗人gFaP抗原亲和纯化多克隆抗体 小鼠抗神经元特异的b-iii微管蛋白单克隆抗体 小鼠抗少突胶质细胞标志物o4单克隆抗体 神经祖细胞多能性的验证： 利用Human/Mouse/rat Neural lineage Functional
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
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