- ¥30400
- Cell Signaling Technology
- 75676
- 美国
- 2025年09月22日
- IP
- 兔
- all
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
美国CST中国公司
- 库存：
1 kit
- 靶点：
多条信号通路
- 克隆性：
多克隆
- 抗原来源：
合成的基序肽段
- 保质期：
24个月
- 抗体英文名：
PTMScan® Multi-Pathway Enrichment Kit
- 抗体名：
信号通路全景筛查试剂盒
- 宿主：
兔
- 适应物种：
all
- 免疫原：
合成的基序肽段
- 形态：
液体
- 应用范围：
IP
- 保存条件：
-20度
- 规格：
1 kit（10 assays）
经过多年的辛苦研发，CST 科学家研发出一款基于质谱的信号通路全景筛查试剂盒 #75676 PTMScan® Multi-Pathway Enrichment Kit 。它自面市以来便享有稳、准、狠的美誉：稳，通路筛查结果可靠；准，允许对一组确定的蛋白质位点以及信号转导节点进行靶向筛选和定量分析；狠，通量极高，且其筛选和定量分析的蛋白质位点和信号转导节点对药物发现、生物标记物发现或诊断学发展至关重要。
#75676 PTMScan® Multi-Pathway Enrichment kit，如下图1。
图1 #75676 产品基本信息
该试剂盒主要是由偶联在琼脂糖珠上的抗体及对应的 IP buffer 组成。该试剂盒中的 “Beads” 是实现后续多通路富集实验的核心和关键。这个 “Beads” 的特别之处就是偶联了几千种 CST 高质量的抗体，包含总蛋白的抗体和磷酸化的抗体。既然是用抗体来做实验，那么实验操作就不难。大概的步骤包括样本总肽段制备及纯化—总肽段与抗体孵育的 IP 实验（多通路多肽富集）—富集后多肽的纯化—LC-MS/MS 实验。若需要更详细的实验步骤，可阅读文章 PTMScan® 试剂盒操作方法和关键环节介绍。
试剂盒包含的抗体可富集并定性定量至少 800 种已知的蛋白和至少 4000 个磷酸化位点，涵盖 19 条经典的信号通路，是样本中靶蛋白高通量筛选和检测的稳健方法。有没有一下子明白用这个抗体做一次 IP-MS，相当于做了上千个 WB 呢？
以 PI3 Kinase/Akt Signaling 为例，蓝色高亮的靶点都可被监测，如下图 2
图2，Akt 信号通路覆盖的靶点
进一步聚类分析的结果如下图（图 3）：
图 3，肝脏、大脑及胚胎组织中各信号通路中多肽靶点分布（左）， AKT 信号通路中特异多肽分布细节图（右）。数据计算的范围是 0（白色，最低富集）-1（蓝色，最高富集）。
运用 PTM Scan® Multi-Pathway Enrichment kit, CST 科学家在人的癌症细胞中通过LC-MS/MS 实验分析了各蛋白分布，最后总结了如下结果图（图4），说明人的癌症细胞中的蛋白磷酸化程度还是很高的，因为激酶的比例占到近1/3（如图4）。该试剂盒提供了对信号通路的综合分析，减少了丢失重要数据点的可能性，可灵活定制后续有针对性的实验。
图 4. 通过 LC-MS/MS 实验分析人的癌症细胞中各蛋白分布
最后我们列出该试剂盒覆盖的所有蛋白位点，以饷大家。
| 4E-BP1 (Ser35) |
4E-BP1 (Ser44) |
4E-BP1 (Thr36) |
4E-BP1 (Thr37) |
| 4E-BP1 (Thr41) |
4E-BP1 (Thr45) |
4E-BP1 (Thr46) |
4E-BP1 (Thr50) |
| 4E-BP1 (Tyr34) |
4E-BP1 (Tyr54) |
4E-BP2 (Ser44) |
4E-BP2 (Thr36) |
| 4E-BP2 (Thr37) |
4E-BP2 (Thr41) |
4E-BP2 (Thr45) |
4E-BP2 (Thr46) |
| 4E-BP2 (Thr50) |
4E-BP2 (Tyr34) |
4E-BP2 (Tyr54) |
4E-BP3 (Thr23) |
| 4E-BP3 (Thr32) |
53BP2 (Tyr610) |
A-Raf (Ser157) |
A-Raf (Ser299) |
| A-Raf (Tyr155) |
A-Raf (Tyr296) |
A-Raf (Tyr302) |
a-Tublin |
| Abi-2 (Ser216) |
Abi-2 (Tyr213) |
Abi-2 iso2 (Ser210) |
Abi-2 iso2 (Tyr207) |
| Abl (Ser113) |
Abl (Tyr115) |
Abl iso2 (Ser132) |
Abl iso2 (Tyr134) |
| Ack (Ser867) |
Ack (Tyr859) |
Ack (Tyr860) |
Ack iso3 (Ser945) |
| Ack iso3 (Tyr937) |
Ack iso3 (Tyr938) |
AFAP1L2 (Tyr54) |
AFAP1L2 (Tyr56) |
| AKNA (Ser997) |
Akt1 (Ser473) |
Akt1 (Thr305) |
Akt1 (Thr308) |
| Akt1 (Thr312) |
Akt1 (Thr479) |
Akt1 (Tyr474) |
Akt2 |
| Akt2 (Ser474) |
Akt2 (Ser476) |
Akt2 (Ser478) |
Akt2 (Thr306) |
| Akt2 (Thr309) |
Akt2 (Thr313) |
Akt2 (Tyr122) |
Akt2 (Tyr316) |
| Akt2 (Tyr475) |
Akt3 (Ser472) |
Akt3 (Ser474) |
Akt3 (Ser476) |
| Akt3 (Thr302) |
Akt3 (Thr305) |
Akt3 (Tyr473) |
ANP-β (Thr540) |
| ANP-β iso2 (Thr540) |
Arg (Ser159) |
Arg (Tyr161) |
Arg iso4 (Ser123) |
| Arg iso4 (Tyr125) |
Arg iso6 (Ser138) |
Arg iso6 (Tyr140) |
ARHGEF17 (Ser383) |
| ATF-2 (Thr69) |
ATF-2 (Thr71) |
ATF-7 |
ATF-7 (Ser424) |
| ATF-7 (Ser428) |
ATF-7 (Ser429) |
ATF-7 (Ser434) |
ATF-7 (Thr418) |
| ATF-7 (Thr432) |
ATF-7 (Tyr421) |
ATF-7 iso2 (Ser392) |
ATF-7 iso2 (Ser396) |
| ATF-7 iso2 (Ser397) |
ATF-7 iso2 (Ser402) |
ATF-7 iso2 (Thr386) |
ATF-7 iso2 (Thr400) |
| ATF-7 iso2 (Tyr389) |
ATG2A (Tyr1314) |
ATM (Ser1981) |
ATM (Ser1988) |
| Aurora A (Thr287) |
Aurora A (Thr288) |
Aurora A (Thr292) |
Aurora B (Thr232) |
| Aurora B (Thr236) |
Aurora C (Thr198) |
Aurora C (Thr202) |
Aurora C iso2 (Thr164) |
| Aurora C iso2 (Thr168) |
Axl (Tyr702) |
Axl (Tyr703) |
b-Actin |
| B-Raf (Ser446) |
B-Raf (Ser447) |
B-Raf (Thr440) |
Bad (Ser71) |
| Bad (Ser74) |
Bad (Ser75) |
Bad (Ser91) |
Bad (Thr80) |
| Bad (Tyr76) |
BAG3 |
BAG3 (Tyr86) |
BAG3 (Tyr93) |
| Bcr/Abl (Ser773) |
Bcr/Abl (Tyr775) |
Bcr/Abl iso2 (Ser627) |
Bcr/Abl iso2 (Tyr629) |
| BLM (Ser74) |
BMPR2 (Ser547) |
BMPR2 (Ser548) |
BMPR2 (Ser549) |
| BMPR2 (Tyr546) |
BMPR2 (Tyr551) |
Caspase-3 (Asp175) |
Caspase-3 (Ser176) |
| Caveolin-1 (Thr15) |
Caveolin-1 (Tyr14) |
Caveolin-1 (Tyr25) |
Caveolin-1 (Tyr6) |
| CBP (Ser1578) |
CCNB1 |
CD28 (Tyr191) |
CD2AP (Ser542) |
| CD2AP (Ser546) |
CD2AP (Ser550) |
CD2AP (Ser553) |
CD2AP (Ser554) |
| CD2AP (Thr539) |
CD2AP (Thr551) |
CD2AP (Tyr541) |
CD2AP (Tyr548) |
| CD45 (Tyr705) |
Cdc25C (Ser168) |
Cdc25C iso3 (Ser125) |
CDK1 |
| CDK1 (Thr14) |
CDK1 (Tyr15) |
CDK1 (Tyr19) |
CDK2 |
| CDK2 (Thr14) |
CDK2 (Tyr15) |
CDK3 |
CDK3 (Thr14) |
| CDK3 (Tyr15) |
CDK5 (Tyr15) |
CdkL5 (Ser446) |
CdkL5 (Ser465) |
| CdkL5 (Ser468) |
CdkL5 (Tyr171) |
CdkL5 (Tyr614) |
CELSR2 (Tyr2679) |
| CENTD2 (Tyr431) |
CENTD2 iso1 (Tyr191) |
CHERP (Ser902) |
CHERP (Ser904) |
| Chk1 (Ser343) |
Chk1 (Ser345) |
Chk1 (Ser357) |
Chk1 (Thr362) |
| CK1-G2 (Ser32) |
CK1-G3 (Ser31) |
CK1-G3 iso3 (Ser31) |
Claudin-4 (Tyr208) |
| Cortactin (Tyr334) |
Cortactin iso3 (Tyr297) |
CRIP2 (Ser74) |
CRIP2 (Tyr75) |
| CRIP2 (Tyr77) |
CTNNA1 |
CTNNA2 |
CTNNB1 |
| CTNNB1 (Ser552) |
CTNNB1 (Ser675) |
CTNNB1 (Ser680) |
CTNNB1 (Ser681) |
| CTNNB1 (Thr551) |
CTNNB1 (Thr556) |
CTNNB1 (Thr679) |
CTTN |
| DBNDD1 (Ser119) |
DBNDD1 (Ser121) |
DBNDD1 (Thr123) |
DBNL (Tyr162) |
| DBNL iso2 (Tyr162) |
DBNL iso3 (Tyr162) |
DDR1 (Tyr792) |
DDR1 (Tyr796) |
| DDR1 (Tyr797) |
DDX29 (Tyr808) |
DDX29 (Tyr811) |
Deptor (Ser279) |
| Deptor (Ser280) |
Deptor (Ser282) |
Deptor (Ser283) |
Deptor (Ser291) |
| Deptor (Ser292) |
Deptor (Ser293) |
Deptor (Ser299) |
Deptor (Thr295) |
| Desmoglein 2 (Ser1059) |
Desmoglein 2 (Ser1060) |
Desmoglein 2 (Ser1068) |
Desmoglein 2 (Thr1056) |
| Desmoglein 2 (Tyr1061) |
Desmoglein 2 (Tyr940) |
Desmoplakin 3 (Ser36) |
Desmoplakin 3 (Ser39) |
| Desmoplakin 3 (Ser665) |
Desmoplakin 3 (Thr21) |
Desmoplakin 3 (Tyr20) |
Desmoplakin 3 (Tyr22) |
| Diaphanous 1 (Tyr128) |
Diaphanous 1 iso2 (Tyr119) |
DNA-PK (Ser893) |
DOT1L (Ser741) |
| DOT1L (Ser753) |
DOT1L (Thr735) |
DOT1L (Thr751) |
DOT1L (Tyr738) |
| DYNLL1 |
DYNLL2 |
DYRK1A (Tyr321) |
DYRK1A iso2 (Tyr312) |
| DYRK1A iso4 (Tyr321) |
DYRK1B (Tyr273) |
DYRK1B iso2 (Tyr273) |
eEF1A1 (Tyr177) |
| eEF1AL3 (Tyr177) |
EGFR (Tyr869) |
EIF1AX |
eIF1AX (Ser74) |
| eIF1AX (Thr73) |
EIF1AY |
eIF1AY (Ser74) |
eIF1AY (Thr73) |
| EIF2A |
EIF2S1 |
EIF4EBP2 |
EIF4H |
| EphB4 (Tyr574) |
EphB4 (Tyr581) |
ERC1 (Ser897) |
ERC1 iso4 (Ser829) |
| Erk1 |
Erk1 (Thr202) |
Erk1 (Tyr204) |
Erk2 |
| Erk2 (Thr185) |
Erk2 (Tyr187) |
Erk3 |
Erk5 (Thr219) |
| Erk5 (Tyr221) |
FAK (Ser574) |
FAK (Thr575) |
FAK (Tyr194) |
| FAK (Tyr570) |
FAK (Tyr576) |
FAK (Tyr577) |
FAK iso2 (Ser422) |
| FAK iso2 (Thr423) |
FAK iso2 (Tyr13) |
FAK iso2 (Tyr418) |
FAK iso2 (Tyr424) |
| FAK iso2 (Tyr425) |
FAK iso5 (Ser574) |
FAK iso5 (Thr575) |
FAK iso5 (Tyr194) |
| FAK iso5 (Tyr570) |
FAK iso5 (Tyr576) |
FAK iso5 (Tyr577) |
FGFR1 (Tyr583) |
| FGFR1 (Tyr585) |
FGFR1 (Tyr653) |
FGFR1 (Tyr654) |
FGFR4 (Tyr642) |
| FGFR4 (Tyr643) |
FGFR4 iso3 (Tyr874) |
FGFR4 iso3 (Tyr875) |
FLVCR1 (Tyr22) |
| FOXK1 (Thr273) |
Fyn (Tyr420) |
Fyn iso2 (Tyr417) |
G-α-s (Tyr190) |
| G-α-s iso2 (Tyr176) |
G-α-s iso3 (Tyr175) |
G-α-s iso4 (Tyr833) |
G-α-s iso5 (Tyr819) |
| Gab2 (Ser474) |
Gab2 (Ser480) |
Gab2 (Ser488) |
Gab2 (Tyr476) |
| GADS (Tyr45) |
GAPDH |
GIT1 (Ser508) |
GIT1 (Tyr510) |
| GIT1 iso3 (Ser521) |
GIT1 iso3 (Tyr523) |
GRB2 |
GSK-3α (Ser20) |
| GSK-3α (Ser21) |
GSK-3α (Ser278) |
GSK-3α (Ser39) |
GSK-3α (Ser41) |
| GSK-3α (Thr19) |
GSK-3α (Tyr279) |
GSK-3β (Ser215) |
GSK-3β (Tyr216) |
| GSK-3β iso2 (Ser215) |
GSK-3β iso2 (Tyr216) |
H3 |
Hck (Ser520) |
| Hck (Tyr411) |
Hck (Tyr51) |
Hck (Tyr522) |
HER3 (Tyr1054) |
| HIPK1 (Ser355) |
HIPK1 (Tyr352) |
HIPK2 (Ser364) |
HIPK2 (Tyr361) |
| HIPK3 (Tyr359) |
hnRNP C1/C2 (Ser121) |
hnRNP C1/C2 iso2 (Ser100) |
hnRNP C1/C2 iso2 (Ser107) |
| hnRNP C1/C2 iso2 (Ser108) |
hnRNP C1/C2 iso2 (Tyr105) |
hnRNP C1/C2 iso4 (Ser100) |
hnRNP C1/C2 iso4 (Ser107) |
| hnRNP C1/C2 iso4 (Ser108) |
hnRNP C1/C2 iso4 (Tyr105) |
HNRPLL (Ser61) |
Hrs (Ser240) |
| Hrs (Thr239) |
Hrs (Thr287) |
Hrs (Tyr286) |
HSP90AA1 |
| HSP90AB1 |
HSPB1 |
HUS1B (Ser121) |
ICK (Thr162) |
| ICK (Tyr159) |
IGF1R (Tyr1161) |
IGF1R (Tyr1165) |
IGF1R (Tyr1166) |
| IkBα (Ser36) |
IkBε (Ser157) |
IkBε iso3 (Ser80) |
IKKγ (Ser376) |
| IKKγ (Ser377) |
IKKγ (Ser383) |
IKKγ (Tyr374) |
InsR (Tyr1185) |
| InsR (Tyr1189) |
InsR (Tyr1190) |
InsR iso2 (Tyr1173) |
InsR iso2 (Tyr1177) |
| InsR iso2 (Tyr1178) |
IRS1 |
IRS-1 (Ser463) |
IRS-1 (Tyr465) |
| IRS-2 (Thr544) |
IRS-2 (Tyr540) |
IRS-2 (Tyr542) |
ITK (Tyr220) |
| JNK1 (Thr183) |
JNK1 (Tyr185) |
JNK1 iso2 (Thr183) |
JNK1 iso2 (Tyr185) |
| JNK2 (Thr183) |
JNK2 (Thr188) |
JNK2 (Tyr185) |
JNK2 iso2 (Thr183) |
| JNK2 iso2 (Thr188) |
JNK2 iso2 (Tyr185) |
JNK2 iso3 (Thr183) |
JNK2 iso3 (Thr188) |
| JNK2 iso3 (Tyr185) |
JNK3 (Thr221) |
JNK3 (Tyr223) |
JNK3 iso2 (Thr221) |
| JNK3 iso2 (Tyr223) |
Jun (Ser73) |
JunD (Ser100) |
JunD (Ser90) |
| KIRREL (Tyr622) |
KIRREL (Tyr625) |
KTN1 (Ser242) |
KTN1 (Ser243) |
| KTN1 (Thr231) |
KTN1 (Thr232) |
KTN1 (Tyr233) |
Lamin A/C (Ser404) |
| Lamin A/C (Ser407) |
Lamin A/C iso2 (Ser404) |
Lamin A/C iso2 (Ser407) |
LARP (Ser293) |
| LARP (Ser627) |
LARP (Ser631) |
LARP (Thr303) |
LARP (Tyr296) |
| LARP (Tyr633) |
LARP iso3 (Ser216) |
LARP iso3 (Ser550) |
LARP iso3 (Ser554) |
| LARP iso3 (Thr226) |
LARP iso3 (Tyr219) |
LARP iso3 (Tyr556) |
LARP iso4 (Ser293) |
| LARP iso4 (Ser627) |
LARP iso4 (Ser631) |
LARP iso4 (Thr303) |
LARP iso4 (Tyr296) |
| LARP iso4 (Tyr633) |
Latrophilin 2 (Tyr1372) |
Latrophilin 2 (Tyr1406) |
Latrophilin 2 (Tyr1407) |
| Latrophilin 2 iso2 (Tyr1316) |
Latrophilin 2 iso2 (Tyr1350) |
Latrophilin 2 iso2 (Tyr1351) |
LBR (Ser159) |
| LBR (Ser167) |
LBR (Tyr161) |
LBR (Tyr166) |
LBR (Tyr22) |
| Lck (Tyr394) |
LEMD2 (Ser499) |
LEMD2 (Ser501) |
LISCH (Ser321) |
| LISCH (Ser326) |
LISCH (Ser332) |
LISCH (Thr327) |
LISCH (Tyr323) |
| LISCH (Tyr328) |
LISCH (Tyr372) |
LMO7 (Ser318) |
LMO7 (Ser322) |
| LMO7 (Tyr323) |
LMO7 iso2 (Ser318) |
LMO7 iso2 (Ser322) |
LMO7 iso2 (Tyr323) |
| LMO7 iso3 (Ser318) |
LMO7 iso3 (Ser322) |
LMO7 iso3 (Tyr323) |
LPP (Tyr273) |
| LPP (Tyr275) |
LRP6 (Ser1490) |
LRP6 (Thr1479) |
LRP6 (Thr1493) |
| LRP6 (Tyr1480) |
LRRK1 (Ser360) |
LRRK1 (Thr358) |
Lyn (Tyr397) |
| Lyn iso2 (Tyr376) |
Lyric (Tyr364) |
Lyric iso3 (Tyr364) |
MAP1B (Ser1171) |
| MAP1B (Ser1322) |
MAP1B (Ser1324) |
MAP1B (Ser1326) |
MAP1B (Ser1330) |
| MAP1B (Ser1339) |
MAP1B (Ser1396) |
MAP1B (Ser1400) |
MAP1B (Ser1406) |
| MAP1B (Ser1412) |
MAP1B (Ser1443) |
MAP1B (Ser1869) |
MAP1B (Ser1899) |
| MAP1B (Ser1915) |
MAP1B (Ser1917) |
MAP1B (Ser1922) |
MAP1B (Ser1939) |
| MAP1B (Ser1956) |
MAP1B (Ser1973) |
MAP1B (Ser2007) |
MAP1B (Ser2024) |
| MAP1B (Thr1334) |
MAP1B (Thr1864) |
MAP1B (Thr1898) |
MAP1B (Thr1925) |
| MAP1B (Thr1964) |
MAP1B (Thr2010) |
MAP1B (Thr2011) |
MAP1B (Thr2034) |
| MAP1B (Tyr1170) |
MAP1B (Tyr1174) |
MAP1B (Tyr1336) |
MAP1B (Tyr1337) |
| MAP1B (Tyr1410) |
MAP1B (Tyr1454) |
MAP1B (Tyr1870) |
MAP1B (Tyr1872) |
| MAP1B (Tyr1904) |
MAP1B (Tyr1905) |
MAP1B (Tyr1906) |
MAP1B (Tyr1921) |
| MAP1B (Tyr1923) |
MAP1B (Tyr1938) |
MAP1B (Tyr1940) |
MAP1B (Tyr1955) |
| MAP1B (Tyr1972) |
MAP1B (Tyr1974) |
MAP1B (Tyr2006) |
MAP1B (Tyr2023) |
| MAP1B (Tyr2040) |
MAP1B (Tyr2042) |
MAP1B (Tyr2057) |
MAP1B (Tyr2059) |
| MAP4 (Tyr47) |
MAP4 iso5 (Tyr47) |
MAP4 iso9 (Tyr47) |
MCM2 (Ser139) |
| MCM2 (Tyr137) |
MEK1 (Ser218) |
MEK1 (Ser222) |
MEK2 (Ser222) |
| MEK2 (Ser226) |
MEKK2 (Ser164) |
MEKK2 (Tyr169) |
Mer (Tyr749) |
| Mer (Tyr753) |
Mer (Tyr754) |
Met (Ser1236) |
Met (Tyr1234) |
| Met (Tyr1235) |
MINK (Tyr505) |
MINK (Tyr507) |
MINK iso4 (Tyr505) |
| MINK iso4 (Tyr507) |
MKK3 (Ser253) |
MKP-1 (Ser359) |
MKP-1 (Ser364) |
| MKP-1 (Ser366) |
MLF1 (Ser34) |
MLK4 (Tyr1028) |
MRCKa (Ser1545) |
| MRCKa (Tyr1544) |
MRCKa iso2 (Ser1567) |
MRCKa iso2 (Tyr1566) |
MRCKa iso3 (Ser1451) |
| MRCKa iso3 (Tyr1450) |
MSK1 (Ser376) |
MSK1 (Ser381) |
MSK1 (Tyr375) |
| MSK2 (Ser360) |
MSK2 (Ser365) |
MSK2 (Tyr359) |
MSK2 iso2 (Ser360) |
| MSK2 iso2 (Ser365) |
MSK2 iso2 (Tyr359) |
MST3 (Thr182) |
MST3 (Thr184) |
| MST3 (Thr190) |
MST3 (Thr194) |
MST3 iso2 (Thr170) |
MST3 iso2 (Thr172) |
| MST3 iso2 (Thr178) |
MST3 iso2 (Thr182) |
MTHFD1 |
mTOR (Ser2442) |
| mTOR (Ser2448) |
mTOR (Ser2450) |
mTOR (Ser2454) |
mTOR (Thr2444) |
| mTOR (Thr2446) |
mTOR (Tyr2449) |
MUC1 (Tyr1218) |
MUC1 (Tyr1229) |
| MUC1 iso2 (Tyr227) |
MUC1 iso2 (Tyr238) |
MUC1 iso6 (Tyr193) |
MUC1 iso6 (Tyr204) |
| MYC |
MYO1E (Ser980) |
MYO1E (Ser983) |
MYO1E (Tyr7) |
| MYO1E (Tyr9) |
MYO1E (Tyr971) |
MYO1E (Tyr974) |
MYPT1 (Ser888) |
| MYPT1 (Ser903) |
MYPT1 (Thr892) |
MYPT1 (Tyr890) |
MYPT1 (Tyr911) |
| MYPT1 (Tyr913) |
MYST4 (Ser1048) |
MYST4 iso4 (Ser1048) |
NF-κB p105 (Ser944) |
| NF-κB p105 (Thr943) |
NF-κB p105 (Thr950) |
Notch1 |
Notch1 (Ser2136) |
| NuMA-1 (Tyr1774) |
Numb (Tyr15) |
Numb iso2 (Tyr15) |
Numb iso3 (Tyr15) |
| NumbL (Tyr56) |
OSR1 (Ser263) |
OSR1 (Thr185) |
OSR1 (Thr189) |
| P130 Cas (Thr385) |
P130 Cas (Tyr115) |
P130 Cas (Tyr128) |
p38α (Thr180) |
| p38α (Tyr182) |
p38α iso2 (Thr180) |
p38α iso2 (Tyr182) |
p38δ (Thr180) |
| p38δ (Tyr182) |
p38γ (Thr183) |
p38γa (Tyr185) |
p53 (Ser392) |
| p70 S6K (Ser243) |
p70 S6K (Thr248) |
p70 S6K (Thr250) |
p70 S6K (Thr252) |
| p70 S6K (Thr256) |
p70 S6K iso2 (Ser220) |
p70 S6K iso2 (Thr225) |
p70 S6K iso2 (Thr227) |
| p70 S6K iso2 (Thr229) |
p70 S6K iso2 (Thr233) |
p70 S6Kb (Ser219) |
p70 S6Kb (Thr226) |
| p70 S6Kb (Thr228) |
p90RSK |
p90RSK (Ser221) |
p90RSK (Thr225) |
| p90RSK (Thr573) |
p90RSK (Tyr220) |
p90RSK (Tyr228) |
p90RSK (Tyr576) |
| p90RSK iso3 (Ser230) |
p90RSK iso3 (Thr234) |
p90RSK iso3 (Thr582) |
p90RSK iso3 (Tyr229) |
| p90RSK iso3 (Tyr237) |
p90RSK iso3 (Tyr585) |
PAK1IP1 (Ser365) |
PAK6 (Ser132) |
| PAR-4 (Ser211) |
PAR-4 (Ser212) |
PAR-4 (Tyr213) |
PARD3 (Tyr388) |
| PARD3 iso10 (Tyr388) |
PARD3 iso5 (Tyr344) |
PARP1 |
PCMT1 (Tyr213) |
| PCTAIRE1 (Tyr176) |
PCTAIRE1 iso3 (Tyr250) |
PCTAIRE2 (Tyr203) |
PDGF Receptor α |
| PDGF Receptor α (Ser566) |
PDGF Receptor α (Ser755) |
PDGF Receptor α (Ser847) |
PDGF Receptor α (Tyr1018) |
| PDGF Receptor α (Tyr572) |
PDGF Receptor α (Tyr574) |
PDGF Receptor α (Tyr742) |
PDGF Receptor α (Tyr754) |
| PDGF Receptor α (Tyr849) |
PDK1 (Ser241) |
PDK1 (Thr245) |
PDK1 iso4 (Ser114) |
| PDK1 iso4 (Thr118) |
PHC3 (Ser616) |
PHC3 (Thr609) |
PHC3 (Thr614) |
| PHC3 (Thr621) |
PHKA1 (Ser729) |
PHKA1 (Ser735) |
PHKA1 (Tyr75) |
| PHKB (Tyr29) |
PHKB iso2 (Tyr22) |
PHKB iso3 (Tyr29) |
PIK3C2A (Ser409) |
| PIK3CA (Ser507) |
PIK3CA (Tyr644) |
PIK3CB (Tyr425) |
PIK3R1 |
| PIK3R1 (Tyr467) |
PIK3R2 |
PIK3R2 (Tyr464) |
PIK3R3 |
| PIK3R3 (Tyr199) |
PIP5K (Tyr1772) |
PITSLRE (Tyr449) |
PITSLRE B (Tyr437) |
| PITSLRE B iso10 (Tyr93) |
PITSLRE iso12 (Tyr93) |
PKAR2A (Tyr385) |
PKCα |
| PKCα (Thr48) |
PKCβ |
PKCβ (Thr48) |
PKCβ (Thr500) |
| PKCβ (Thr504) |
PKCβ iso2 (Thr48) |
PKCβ iso2 (Thr500) |
PKCβ iso2 (Thr504) |
| PKCδ (Ser645) |
PKCδ (Ser647) |
PKCδ (Ser654) |
PKCδ (Ser664) |
| PKCδ (Tyr646) |
PKCδ iso13 (Ser645) |
PKCδ iso13 (Ser647) |
PKCδ iso13 (Ser654) |
| PKCδ iso13 (Ser664) |
PKCδ iso13 (Tyr646) |
PKCQ |
PKD2 (Ser375) |
| PKD2 (Tyr378) |
PKN1 (Ser916) |
PKN2 (Ser306) |
PLCγ1 (Ser1248) |
| PLCγ1 (Ser470) |
PLCγ1 (Thr50) |
PLCγ1 (Tyr472) |
PLCγ1 iso2 (Ser1249) |
| PLCγ1 iso2 (Ser470) |
PLCγ1 iso2 (Thr50) |
PLCγ1 iso2 (Tyr472) |
PLEKHA2 (Ser182) |
| PLEKHA2 (Ser184) |
PLEKHA2 (Tyr185) |
PLEKHA5 (Tyr590) |
PLEKHG4 (Ser677) |
| PLEKHG4 (Tyr676) |
PPP1CB |
PPP1CB (Tyr304) |
PPP1CB (Tyr306) |
| PPP2R5D (Tyr451) |
PRAS40 (Thr246) |
PRAS40 iso3 (Thr266) |
PRC1 (Ser513) |
| PRC1 (Tyr511) |
PRC1 iso2 (Ser483) |
PRC1 iso2 (Tyr481) |
PRC1 iso3 (Ser472) |
| PRC1 iso3 (Tyr470) |
PRP4 (Tyr849) |
PSMC4 |
PTP-PEST (Tyr88) |
| PTPN13 (Tyr943) |
PTPN13 iso3 (Tyr943) |
PTPRA (Tyr295) |
PTPRA (Tyr798) |
| PTPRA iso2 (Tyr286) |
PTPRA iso2 (Tyr789) |
PTPRD (Tyr1699) |
PTPRD iso5 (Tyr1292) |
| PTPRD iso7 (Tyr1283) |
PTPRE (Tyr696) |
PTPRE iso2 (Tyr638) |
PTPRG (Tyr906) |
| PTPRG (Tyr911) |
PTPRJ (Thr339) |
PTPRK (Tyr940) |
PTPRM (Tyr953) |
| PTPRS (Tyr1735) |
Pyk2 (Tyr579) |
Pyk2 (Tyr580) |
Pyk2 iso2 (Tyr579) |
| Pyk2 iso2 (Tyr580) |
RAB23 (Ser186) |
RAB23 (Ser187) |
RAB23 (Ser188) |
| RAB23 (Thr184) |
Rab7 (Tyr183) |
RABEP1 (Tyr501) |
RABEP1 iso2 (Tyr501) |
| Raf1 (Ser338) |
Raf1 (Ser339) |
Raf1 (Tyr340) |
Raf1 (Tyr341) |
| RAIG1 (Ser321) |
RAIG1 (Ser345) |
RAIG1 (Thr322) |
RAIG1 (Tyr317) |
| RAIG1 (Tyr347) |
RAIG1 (Tyr350) |
Rap1GAP (Thr23) |
Rap1GAP (Tyr27) |
| Rap1GAP iso2 (Thr23) |
Rap1GAP iso2 (Tyr27) |
Rap1GAP iso3 (Thr54) |
Rap1GAP iso3 (Tyr58) |
| Rap1GAP iso5 (Thr87) |
Rap1GAP iso5 (Tyr91) |
RAPGEF2 (Ser960) |
RAPGEF2 iso2 (Ser948) |
| RAPH1 (Ser822) |
RAPH1 (Thr830) |
RAPH1 (Tyr524) |
RAPH1 (Tyr823) |
| Rb (Ser773) |
Rb (Ser780) |
Rb (Ser788) |
Rb (Ser794) |
| Rb (Ser795) |
Rb (Thr774) |
Rb (Thr778) |
Rb (Tyr771) |
| Rb (Tyr790) |
RBBP6 (Ser516) |
RbBP6 iso2 (Ser516) |
RBM14 |
| RBM14 (Ser215) |
RBM14 (Ser220) |
RBM14 (Ser225) |
RBM14 (Tyr226) |
| RBM34 (Tyr71) |
RBM47 (Ser540) |
RBM47 (Thr533) |
RBM47 (Thr554) |
| RBM47 (Tyr537) |
RBM47 (Tyr541) |
RBMS2 (Ser277) |
RBMS2 (Ser280) |
| RBMS2 (Ser285) |
RBMS2 (Tyr290) |
RhoA (Tyr34) |
RhoA iso3 (Tyr34) |
| RhoG (Tyr32) |
RICS (Tyr2048) |
Rictor (Ser1320) |
Rictor (Tyr1269) |
| Rin1 (Thr696) |
Rin1 (Tyr36) |
Rin1 (Tyr698) |
Rin1 (Tyr701) |
| RIPK1 |
RIPK2 (Ser176) |
RPS3A |
RSK2 |
| RSK2 (Ser227) |
RSK2 (Thr231) |
RSK2 (Thr577) |
RSK2 (Tyr226) |
| RSK2 (Tyr234) |
RSK2 (Tyr580) |
RSK3 |
RSK4 |
| RSK4 (Ser232) |
RSK4 (Thr236) |
RSK4 (Tyr231) |
RSK4 (Tyr239) |
| S6 (Ser235) |
S6 (Ser236) |
S6 (Ser240) |
S6 (Ser242) |
| S6 (Ser244) |
S6 (Ser246) |
S6 (Ser247) |
S6 (Thr241) |
| SBNO1 (Ser815) |
SBNO1 (Ser817) |
SBNO1 (Ser818) |
SBNO1 (Ser823) |
| SBNO1 (Ser837) |
SBNO1 (Thr819) |
SBNO1 (Thr835) |
SEC6L1 (Tyr737) |
| SF3A1 (Ser413) |
SF3A1 (Ser423) |
SF3A1 (Thr416) |
SgK223 (Ser490) |
| Shb (Tyr268) |
Shb (Tyr272) |
SHIP-2 (Tyr886) |
SHP-1 (Tyr301) |
| SHP-1 iso5 (Tyr301) |
SHP-2 (Ser302) |
SHP-2 (Tyr304) |
SIPA1L1 (Ser1632) |
| SIPA1L1 (Tyr1633) |
SLITRK4 (Tyr811) |
SLITRK5 (Tyr749) |
SLITRK5 (Tyr751) |
| SLK (Tyr1225) |
SMAD1 |
Smad1 (Ser462) |
Smad1 (Ser463) |
| Smad1 (Ser465) |
Smad2 |
Smad3 |
Smad5 |
| Smad5 (Ser463) |
Smad5 (Ser465) |
Smad8 (Ser464) |
Smad8 (Ser465) |
| Smad8 (Ser467) |
Smad9 |
SMG1 (Ser147) |
SOS2 (Ser1132) |
| Src (Tyr419) |
STAM2 (Ser372) |
STAM2 (Ser375) |
STAM2 (Tyr371) |
| STAM2 (Tyr374) |
Stat1 |
Stat3 |
Stat3 (Ser691) |
| Stat3 (Ser701) |
Stat3 (Thr708) |
Stat3 (Tyr705) |
Stat3 iso3 (Ser691) |
| Stat3 iso3 (Ser701) |
Stat3 iso3 (Thr708) |
Stat3 iso3 (Tyr705) |
Stat5A (Tyr694) |
| Stat5B (Tyr699) |
Stat6 (Tyr641) |
STLK3 (Ser309) |
STLK3 (Thr231) |
| STLK3 (Thr235) |
Syk (Tyr525) |
Syk (Tyr526) |
TANC2 (Tyr1919) |
| TANC2 (Tyr1920) |
TBC1D1 (Ser237) |
Tec (Tyr228) |
Tec (Tyr233) |
| Tensin 1 (Tyr766) |
TIE1 (Ser472) |
Timeless (Ser1149) |
TMOD3 (Tyr86) |
| TMPO (Ser388) |
TMPO (Tyr390) |
TMPO (Tyr394) |
TMPO iso2 (Ser279) |
| TMPO iso2 (Tyr281) |
TMPO iso2 (Tyr285) |
TPD52L2 |
TPD52L2 (Ser96) |
| TPD52L2 (Tyr106) |
TPX2 (Ser322) |
TPX2 (Thr323) |
TPX2 (Thr338) |
| TPX2 (Tyr324) |
TSG118 (Tyr255) |
TSSC4 (Ser143) |
TSSC4 (Ser146) |
| TSSC4 (Tyr155) |
Tyro3 (Tyr681) |
Tyro3 (Tyr685) |
Tyro3 (Tyr686) |
| ULK4 (Thr134) |
WDR32 (Ser61) |
WDR32 (Ser63) |
WNK1 (Thr1963) |
| WNK1 iso4 (Thr2215) |
YAP1 (Tyr407) |
YAP1 iso2 (Tyr391) |
Yes (Tyr426) |
| YSK1 (Thr166) |
YSK1 (Thr168) |
YSK1 (Thr174) |
YSK1 (Thr178) |
| ZAP3 (Ser561) |
ZAP3 iso4 (Ser756) |
ZAP-70 (Ser289) |
ZAP-70 (Ser301) |
| ZAP-70 (Ser313) |
ZAP-70 (Ser317) |
ZAP-70 (Ser320) |
ZAP-70 (Thr494) |
| ZAP-70 (Tyr292) |
ZAP-70 (Tyr315) |
ZAP-70 (Tyr319) |
ZAP-70 (Tyr492) |
| ZAP-70 (Tyr493) |
ZC3H15 (Ser135) |
ZC3H15 (Tyr137) |
ZNF598 (Ser288) |
| ZNF598 iso2 (Ser288) |
ZNF828 (Ser405) |
ZNF828 (Thr403) |
ZO-1 (Ser1360) |
| ZO-1 (Tyr1354) |
ZO-1 (Tyr1355) |
ZO-1 (Tyr1361) |
ZO-1 iso2 (Ser1280) |
| ZO-1 iso2 (Tyr1274) |
ZO-1 iso2 (Tyr1275) |
ZO-1 iso2 (Tyr1281) |
Zyxin (Ser169) |
| Zyxin (Ser170) |
Zyxin (Tyr172) |
||
参考文献：
1. Stokes,M.P. et al. (2013) Quantitative pro ling of DNA damage and apoptotic pathwaysin UV damaged cells using PTMScan Direct. Int. J. Mol. Sci. 14(1), 286–307.
2. Pease,B.N. etal. (2013) Global Analysis of Protein Expres- sion and Phosphorylationof Three Stages of Plasmodium falciparum Intraerythrocytic Development. J.Proteome Res. 2(9):4028–4045.
3. Stokes,M.P. et al. (2012) PTMScan Direct: identi cation and quanti cation of peptidesfrom critical signaling proteins by immunoa nity enrichment coupled withLC-MS/MS. Mol. Cell Proteomics 11(5), 187–201.
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文献和实验实验原理 Add a mixture of biotinylated monoclonal antibodies against non-DC cells to your starting sample. Add Depletion MyOne™ SA Dynabeads® and allow them to bind to the non-DCs during a short incubation. Separate
Dynabeads® Co-Immunoprecipitation Kit
Dynabeads® Co-Immunoprecipitation Kit Magnet: e.g. DynaMag™-2 Mixer allowing rotation or tilting of tubes. Antibodies of your choice. 实验步骤 Day 1 1. Weigh out the appropriate
of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
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