Phospho-Tyrosine Mouse mAb (P-

Product Pathways - Motif Antibodies

• more info
• application references
• datasheet PDF
• MSDS PDF
• protocols

Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411

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Applications	Reactivity	Sensitivity	Isotype
W IP IHC-P IF-F IF-IC IF-P F E-P	All	Endogenous	Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  IF-P=Immunofluorescence (Paraffin)  F=Flow Cytometry  E-P=ELISA (Peptide)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

9411:

ELISA Peptide , Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides .

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

1. Schlessinger, J. (2000) Cell 103, 211-25.
2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

• Smith, J.A. et al. (2005) Cancer Res 65, 1027-34. Applications: Western Blotting
• Ye, Z.J. et al. (2005) Proc Natl Acad Sci U S A 102, 18461-6. Applications: Western Blotting
• Sharma, A. et al. (2003) Mol Cell Proteomics 2, 1217-24. Applications: IP Western Blotting
• Li, Y. et al. (2003) Mol Cell Biol 23, 6255-66. Applications: IP Western Blotting
• Kogata, N. et al. (2003) Mol Biol Cell 14, 3553-64. Applications: Western Blotting
• del Rincón, S.V. et al. (2003) Oncogene 22, 3353-60. Applications: Western Blotting
• Goldberg, G.S. et al. (2003) J Biol Chem 278, 46533-40. Applications: Western Blotting
• Klein, R.D. and Fischer, S.M. (2002) Carcinogenesis 23, 217-21. Applications: Western Blotting
• Perez, O.D. and Nolan, G.P. (2002) Nat Biotechnol 20, 155-62. Applications: Flow Cytometry
• Kaluz, S. et al. (2002) Cancer Res 62, 4469-77. Applications: IP
• Kato, R. et al. (2002) Clin Cancer Res 8, 2455-62. Applications: IP
• Asao, H. et al. (2001) J Immunol 167, 1-5. Applications: Western Blot
• Stewart, D.M. et al. (2001) Endocrinology 142, 98-107. Applications: Western Blotting
• Farhang-Fallah, J. et al. (2000) J Biol Chem 275, 40492-7. Applications: Western Blot
• Chang, C.I. et al. (2000) J Immunol 165, 2134-41. Applications: Western Blot

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 9414 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor^® 488 Conjugate)
• 9415 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Alexa Fluor^® 647 Conjugate)
• 9416 Phospho-Tyrosine Mouse mAb (P-Tyr-102)
• 9417 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)
• 9419 Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)
• 9386 Phospho-Threonine (42H4) Mouse mAb
• 9614 Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb
• 9624 Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb
• 8102 SignalSlide^® Phospho-EGF Receptor IHC Controls
• 8112 SignalStain^® Antibody Diluent
• 7076 Anti-mouse IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8125 SignalStain^® Boost IHC Detection Reagent (HRP, Mouse)
• 5425 Normal Goat Serum
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)

License/Use Restrictions: Use of CST Motif Antibodies within certain methods (e.g., U.S. Patent No.'s 7,198,896 & 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

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For Research Use Only. Not For Use In Diagnostic Procedures.