- 询价
- Cell Signaling Technology
- 9751
- USA
- 2025年07月15日
- W, IHC-P, IF-IC, ChIP
- H,M,R,Mk,Dm,Sc,X,Z
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
- 抗原：
synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated
- 应用范围：
W, IHC-P, IF-IC, ChIP
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk,Dm,Sc,X,Z
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
单克隆
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | 询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | 询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | 询价 |
20190715-0926 期间，购买任一ChIP试剂盒，可0元得该 T(20ul) 包装抗体或礼品！
促销活动详情：
促销时间
2019年7月15日—2019年9月26日
优惠一
购买任一ChIP试剂盒（见下表1），将特别赠送经ChIP或ChIP-seq验证的小包装抗体一支（见下表2）或精美礼品一份（见优惠二）。
促销试剂盒列表1
| 货号 | 产品名称 | 应用 |
| 56383S | SimpleChIP® Plus Sonication Chromatin IP Kit（超声法，磁珠） | ChIP ChIP-seq |
| 9005S | SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads)（酶解法，磁珠） | ChIP ChIP-seq |
| 9004S | SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads)（酶解法，琼脂糖微珠） | ChIP |
| 9003S | SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)（酶解法，磁珠） | ChIP ChIP-seq |
| 9002S | SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)（酶解法，琼脂糖微珠） | ChIP |
| 56795S | SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina®（DNA 文库构建） | ChIP-seq |
赠送抗体列表2
优惠二
一次性购买促销抗体（优惠一中6个ChIP试剂盒除外）目录价格达7000元，即可获赠精美礼品一份。
查询参加促销的具体产品，点击此处或微信公众号「CST博士互助平台」后台回复“促销” ，了解促销详情。
#9751T Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb 产品详情：
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dm=D. melanogaster X=Xenopus Z=Zebrafish Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC ChIP | H M R Mk Dm Sc (X) (Z) | Endogenous | 17 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Tri-Methyl-Histone H3 (Lys4) Antibody detects endogenous levels of histone H3 when tri-methylated on Lys4. This antibody shows some cross-reactivity with histone H3 that is di-methylated on Lys4, but does not cross-react with non-methylated or mono-methylated histone H3 Lys4. In addition, the antibody does not cross-react with methylated histone H3 Lys9, Lys27, Lys36 or methylated histone H4 Lys20. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys4 is tri-methylated. Western Blotting
Antibody specificity was determined by Western blotting. HeLa and NIH/3T3 cell lysates were probed with Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb (Panel A) or Tri-Methyl Histone H3 (Lys4) Rabbit mAb pre-adsorbed with 1.5 μM of various competitor peptides (Panels B-M). As shown, only the tri-methyl histone H3 (Lys4) peptide competed away binding of the antibody. Western Blotting
Western blot analysis of various cell types using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Tri-Methyl Histone H3 (Lys4) (C42D8) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1). Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development (2,3). Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4) (4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing (4). Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5) (5-8). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker (9).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验法所替代。利用微球菌核酸酶的切割特性（在染色质核小体之间的 DNA 更容易被切开），可以快速温和地将染色质 DNA 切断，且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态，使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒，含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体（Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620）、阴性对照抗体、DNA
实际上将作为阴性对照使用。CST 推荐使用 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型（H3、H3.3、CENP-A）。因此，不管检测的位点活性状态如何，该抗体都能为 ChIP 实验提供通用的阳性对照。 阴性对照 阴性对照抗体（如正常兔 IgG）不能识别特异抗原表位，因此可用于检测非特异性结合。例如，如果阴性对照样品中的产物量等于特异
secondary antibody review -- data from 99 publications
data
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