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- C3580-0500
- 2025年12月24日
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- 详细信息
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- 文献和实验
- 技术资料
- 供应商：
上海逍鹏生物科技有限公司
- 库存：
充足
- 英文名：
Phosphate Buffered Salined
- 规格：
500ml
【Product Description】
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first
balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to
prevent calcium precipitation.
The function of a salt solution is:
To maintain the medium within physiological pH range.
To maintain intracellular and extra cellular osmotic balance.
Modified with a carbohydrate, such as glucose serves as an energy source
It is most commonly used for tissue disaggregation and monolayer
dispersal since presence of Calcium and Magnesium ions may hinder the trypsin activity. It is also used for routine
immunohistochemical testing and as a general purpose solution for washing cells in various haematological and molecular biology procedures.
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first
balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to
prevent calcium precipitation.
The function of a salt solution is:
To maintain the medium within physiological pH range.
To maintain intracellular and extra cellular osmotic balance.
Modified with a carbohydrate, such as glucose serves as an energy source
It is most commonly used for tissue disaggregation and monolayer
dispersal since presence of Calcium and Magnesium ions may hinder the trypsin activity. It is also used for routine
immunohistochemical testing and as a general purpose solution for washing cells in various haematological and molecular biology procedures.
【Storage and Stability】
The product should be stored at room temperature (15-30°C). The product should not be left in the light for prolonged periods as it is light-sensitive. When stored in the dark under ideal conditions, the product is stable until the expiry date
【Procedure】
1.Take a bottle from the defined storage conditions at Room Temperature (15-30°C) and read the label.
2. Ensure that the cap of the bottle is tight.
3.Gently swirl the solution in the bottle.
4. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
5. Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
【Quality Control】
Phosphate Buffered Salined is tested for sterility, pH, Osmolality and Endotoxin concentrations.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
The product should be stored at room temperature (15-30°C). The product should not be left in the light for prolonged periods as it is light-sensitive. When stored in the dark under ideal conditions, the product is stable until the expiry date
【Procedure】
1.Take a bottle from the defined storage conditions at Room Temperature (15-30°C) and read the label.
2. Ensure that the cap of the bottle is tight.
3.Gently swirl the solution in the bottle.
4. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
5. Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
【Quality Control】
Phosphate Buffered Salined is tested for sterility, pH, Osmolality and Endotoxin concentrations.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
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文献和实验相关实验
相关专题 因为钙离子和镁离子会影响胰酶的消化能力，导致胰酶消化能力下降，培养细胞时，消化的细胞数量有限，加上细胞的密度依赖性，所以细胞培养时最好使用不含有钙镁离子的PBS 缓冲液。
酚红 Hanks’ 含钙镁， 不含酚红 D- Hanks’ 不含钙镁 含酚红 D- Hanks’ 不含钙镁 不含酚红 DPBS 不含钙镁 不含酚红 PBS 不含钙镁 不含酚红 10*PBS 不含钙镁 不含
含钙镁， 不含酚红 D- Hanks’ 不含钙镁 含酚红 D- Hanks’ 不含钙镁 不含酚红 DPBS 不含钙镁 不含酚红 PBS 不含钙镁 不含酚红 10*PBS 不含钙镁 不含
技术资料暂无技术资料 索取技术资料
PBS（不含钙，镁）
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