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- C3581-0500
- 2026年01月12日
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- 文献和实验
- 技术资料
- 供应商：
上海逍鹏生物科技有限公司
- 库存：
充足
- 英文名：
Phosphate Buffered Salined，SLE Grade（Endotoxin≤0.01EU/ml）
- 规格：
500ml
All media used in tissue culture have a basis of a synthetic mixture of inorganic salts known as a physiological or balanced salt solution (BSS). All the physiological salt solutions have been derived from the salt solution originally described by Sydney Ringer (1885). The first balanced salt solution to be developed specifically for supporting the metabolism of mammalian cells was Tyrode's solution. Since then many modifications have been done to obtain better buffering salt solutions and to prevent calcium precipitation. It is super low endotoxin SLETM grade，Endotoxin≤0.01EU/ml by LAL method. The production process and quality management system meet the requirements of cGMP and has passed ISO13485 certification.
The function of a salt solution is: To maintain the medium within physiological pH range. To maintain intracellular and extra cellular osmotic balance. Modified with a carbohydrate, such as glucose serves as an energy source It is most commonly used for tissue disaggregation and monolayer dispersal since presence of Calcium and Magnesium ions may hinder the trypsin activity. It is also used for routine immunohistochemical testing and as a general purpose solution for washing cells in various haematological and molecular biology procedures.
The product should be stored at room temperature (15-30°C). The product should not be left in the light for prolonged periods as it is light-sensitive. When stored in the dark under ideal conditions, the product is stable until the expiry date
【Procedure】
1.Take a bottle from the defined storage conditions at Room Temperature (15-30°C) and read the label.
2. Ensure that the cap of the bottle is tight.
3.Gently swirl the solution in the bottle.
4. Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
5. Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
【Quality Control】
Phosphate Buffered Salined is tested for sterility, pH, Osmolality and Endotoxin concentrations.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
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文献和实验含钙镁， 不含酚红 D- Hanks’ 不含钙镁 含酚红 D- Hanks’ 不含钙镁 不含酚红 DPBS 不含钙镁 不含酚红 PBS 不含钙镁 不含酚红 10*PBS 不含钙镁 不含
Hanks：平衡盐溶液——无机盐和葡萄糖浓度接近大部分动植物 细胞的水平，可作为动植物细胞短期培养(保存)。 Hank's配方:NaCl 8.01;KCl 0.4;CaCl2 0.14; NaHCO3 0.35;KH2PO 4 0.06;Glucose 0.34(g/l); D-Hank' s:不含钙离子、镁离子、葡萄糖。( T7 e# L7 e, K2 N 相比较而言，hanks液中盐成分较PBS 复杂，且有葡萄糖，可为细胞提供简单的营养，而pbs中只有磷酸根，钠，钾，氯
相关专题 小鼠胚胎成纤维细胞的富集 1、给13-14天的孕鼠注射大约0.5ml阿佛丁。当鼠麻醉后，实施断颈法处死小鼠。 2、用70%乙醇擦拭腹部，把皮肤向后拉，暴露出腹膜。用消毒过的工具，剪开腹壁以暴露出子宫角。将子宫角移到10cm的皿里。用10ml不含钙镁离子的PBS 洗三遍。 3、用剪刀剪开每一测的胚囊,并将胚胎移到培养皿中。 4、用两副钟表镊子将胎盘和膜与胚胎分离开，分离后切除
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