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- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海逍鹏生物科技有限公司
- 库存：
充足
- 英文名：
McCoy's 5A Medium（(Modified)）, with L-Glutamine
- 规格：
500ml
McCoy's 5A medium was developed at Roswell Park Memorial Institute in Buffalo, New York. The first medium was developed in 1955 as the result of studies on the nutritional requirements of the Walker 256 carcinoma. The original formulation was based on the amino acids in concentrations similar to those in Eagle's medium as well as the water soluble vitamins of Medium199. Modifications to the original formulation resulted in the final version being published in 1960. The final formulation also incorporates modifications done by Iwakata and Grace and contains increased amounts of folic acid, vitamin B12, and peptone. This medium is also known to support growth of primary cultures derived from a variety of tissues.
Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.
McCoy's 5A medium（(Modified)）, with L-Glutamine should be stored at 2-8°C. Use before the expiry date.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
McCoy's 5A Medium（(Modified)）, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using Vero.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
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文献和实验x-S的质控图是检验主管技师考试要求掌握的内容，医学教育网搜集整理相关内容供大家参考。 后来Henry和Segalove对X-R质控图进行了修改，他们以20次质控物的单次测定结果，计算其x及标准差（s），以x±2S为警告限，以x±3S为失控限，这也就是现在广为应用的Levey—Jennings质控图，从质控图的体系来说，它属于单值质控图中的x-S质控图。这个质控图适用于质控数据取得需要较长周期或费用昂贵时，其方法比较简单，易于推广。实际上这两种质控图的检定力是不同的，原
Henry和Segalove对X－R质控图进行了修改，他们以20次质控物的单次测定结果，计算其x及标准差（s），以x±2S为警告限，以x±3S为失控限，这也就是现在广为应用的Levey-Jennings质控图，从质控图的体系来说，它属于单值质控图中的x-S质控图。这个质控图适用于质控数据取得需要较长周期或费用昂贵时，其方法比较简单，易于推广。实际上这两种质控图的检定力是不同的，原Levey-Jennings质控图优于用单次测定结果绘制的医学`教育网搜集整理质控图，且提供了更多的质量
含梗率是指烟梗在烟叶中所占的质量分数（%）。烟叶的含梗率受烟叶的类型。品种、烟叶所生长的部位、叶片形状和厚度、梗的粗细等影响。不同品种烟叶含梗率关系为烤烟>白肋烟>香料烟。烤烟含梗率在24-30%，平均为25%,左右。烟叶着生部位不同，含梗率也不同，,中下部叶高于上部叶。由于烟叶从叶基到叶尖，烟梗由粗逐渐变细，打叶复烤工艺利用这一特点，可在打叶前设置切尖工序，以降低打叶设备负荷和减少叶片造碎，提高大中片合格
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