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Sugden labMcArdle Laboratory for Cancer Research University of Wisconsin-Madison Medical School DnaseI Footprinting (Based upon the protocol from the Promega Core Footprinting Kit) 5x Binding Buffer ...

DNA extraction from Mutation Detection Enhancement (MDE) Gel Stained with Silver Nitrate Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand conformational polymorphism ( ...

Recovering DNA from agarose gels Paul N. Hengen Ph.D. (July 14 1999) Introduction ========Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.mo ...

CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION FROM PLANT LEAF / LEAVES SAMPLES (see also DNA RNA double isolation procedure if both DNA and RNA are needed) Reagents ...

NH4Ac and EtOH precipitation of DNA Add NH4Ac (10M stock or solid) to the sample for a final concentration of 2.5M mix (spin at 4℃ transfer the supernatant to a new tube; optional spin for extra puri ...

PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS 1.Cut 10-20X 10μm sections of formalin fixed paraffin samples into eppendorf tubes. 2.Add 1 ml xylene mix incubate at 55℃ for 15mins. Release pressure s ...

Mate ...

Ⅰ.Isolation of Single Stranded DNA 1.Transform plasmid to be mutagenised into JM109. JM109 is endA1 recA1 gyrA96 thi hsdR17(rk- mk+) relA1 supE44 l- d(lac-proAB) traD36 proA+B+ lacIqdM15. JM109 must ...

BUFFERS: 1 ...

1.显性标记与选择效率 一部分分子标记系统如RAPD、AFLP具有显性或部分显性的特点，无法区分基因是纯合还是杂合，不能提供完整的遗传信息。Haley等（1994）提出相斥相（Repulsion－phase）的RAPD标记无论对显性还是隐性均可极大地提高选择效率，他们找到了和菜豆普通花叶病毒抗性基因bc-3连锁相引标记-1，相斥标记bc-2，用标记-1选择纯合抗病株、杂合体、纯合感病株分别占26. ...

磷酸钙-DNA 共沉淀法 核酸以磷酸钙-DNA 共沉淀物的形式出现时，可使DNA 附在细胞表面，利于细胞吞入摄取，或通过细胞膜脂相收缩时裂开的空隙进入细胞内，进入细胞的DNA 仅有1%～5%可以进入细胞核中，其中仅有不到1%的DNA 可以与细胞DNA 整合，在细胞中进行稳定表达，基因转导的频率大约为10-4，这项技术能用于任何DNA 导入哺乳类动物进行暂时性表达或长期转化的研究。此方法对于贴壁细 ...

1.如何测定引物的OD值？ 用紫外分光光度计在260nm波长测定溶液的吸光度来定量，请注意紫外分光光度计的使用，测定时溶液的吸光度最好稀释到0.2-0.8之间(吸光度太高或太低会有较大的误差)。DNA 干粉用一定体积的水充分振荡溶解以后，取部分溶液稀释到1ml并在1ml标准比色皿中测定其吸光度，即为所测体积的OD值，进而可以计算出母液的OD值。 举例：您拿到一管干粉的DNA ，用1ml水溶解成 ...

1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs. 2. Photograph the gel with a ruler adjacent to the molecular weight markers as a reference. 3 ...

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I am having huge problems with bisulfite PCR. Southern analysis indicates that my DNA is unmethylated. My first bisulfite treatment also indicated that the DNA was unmethylated. Subsequent treatments ...

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David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cl ...

Inoculate a 5ml overnight of E.coli in LB+20 mM MgSO4. Next morninginoculate 250 ml LB+20 mM Mg++ in a 2L flask with about 2ml overnight culture.Grow at room temp (23℃)with good aeration (250rpm)to an ...

SOLUTIONS: Green and blue solutions from Clone Checker kit (cat. no. 11666-013). PROCEDURE: Place 3-6µl of an overnight culture in an Eppendorf tube (or spread part of a bacterial colony on the ...

Many ...