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Assemble the following components: •Cellsgrown in 10 cm dishes •Room Temp.PBS •Cold PBS •Cold Sonication Buffer (1% SDS10 mM EDTA50 mM Tris-HCl pH 7.5) Prepare sonication buffe ...

Purification of GST fusion proteins in E.coli GST融合蛋白纯化--纯化小量蛋白 Sugden labMcArdle Laboratory for Cancer ResearchUniversity of Wisconsin-Madison Medical School Small scale fusion protein preparation Gr ...

Buffer A Buffer B ...

主要特点： · 准确灵敏：BCA试剂的蛋白质测定范围是20－2000μg/ml采用加强方法可检测到5μg/ml；MicroBCA试剂测定范围是0.5-20μg/ml。 · 快速：45分钟内完成测定，比经典的Lowry法快4倍而且更加方便。 · 经济实用：除试管外，测定可在微孔板中进行，大大节约样品和试剂用量。 · 不受样品 ...

This method was successful in our lab using prostate tissue and for our specific objectives.Investigators must be aware that they will need to tailor the following protocol for their own research obje ...

J. Movius K Coachman and S. Hahn (Hahn Lab) Induction of BRF in bacteria and purification on Ni-NTA agarose Transform BRF plasmid into strain BL21 DE3 (pLysS) or JM25 (DE3 containing the Arg t ...

Place the tissues on labeled aluminum foil and immediately place in dry ice.It is imperative that the tissues stay cold so that protease do not have time to act on the protein. Place the tissues in a ...

PIC Cross-linking and Immune Precipitation James FishburnHahn Lab March 2006 References Fishburn et.al.2005Molecular Cellvol.18 #3: Experimental Procedures pg.376 Immobilized Template assay (Hahn lab ...

Sample Buffer: 10 ml 0.06M Tris-HCl pH 6.8 0.6 ml 1M Tris 6.8 ...

1.Grow 25mls yeast cells to 5x10E6. 2.Sequentially spin down cells in a 15 ml polypro tube. 3.Wash cells with 1ml ice cold ddH2Otransferrring to an eppy tube. 4.Recon.cells in 1 ml ice cold ddH2O cont ...

HelveticaGenevaSwissSunSans-Regular" size="2"Linda Warfield Hahn Lab 2003 HelveticaGenevaSwissSunSans-Regular" size="2"Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 a ...

Purpose and backgrounds About nuclide... 35-S Decay: (b-ray0.167 MeV) Half life: 87.5 days Thick acrylic shield can block most of the emission.Because of the low energyit is impossible to detect spill ...

HIS-tagged protein purification John Mundy Institute of Molecular Biology Copenhagen Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#his 1 liter cell prep 1) grow 20ml ...

Hancock Laboratory Methods.Department of Microbiology and Immunology University of British ColumbiaBritish ColumbiaCanada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=28 MATERIALS: Running buff ...

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Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples.Moreoverthe development of two-dimensional (2D)gel electrophoresis has provided a tool for di ...

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer Steve Hahn Protein Dilution Buffer 5ml 20 mM Tris pH7.9 100 microliters 1M Tris 7.9 150 mM KCl 0.75 ml 1 M KCl 1 mM DTT 50 microliter ...

Hahn Lab 1.In microfuge tubesspin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells.Remove YT (or LB)media with a pipetman or drawn out pasteur pipette and freez ...

Cell Lysis/Western/IP Steven FinkbeinerDepartments of Neurology and PhysiologyUCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Cell_Lysis.shtml compiled by 6/95 by AJS from AB)Fresh 1 ...

1.Crosslink protein DNA complexes in vivo Grow 50ml yeast culture to OD600 0.5-1.0.In fume hoodadd 1.4ml 37% fomaldehyde solution to a 50ml conical tube.Fill tube with culture to just below the 50ml l ...