全部

J. Movius K Coachman and S. Hahn (Hahn Lab) Induction of BRF in bacteria and purification on Ni-NTA agarose Transform BRF plasmid into strain BL21 DE3 (pLysS) or JM25 (DE3 containing the Arg t ...

Place the tissues on labeled aluminum foil and immediately place in dry ice.It is imperative that the tissues stay cold so that protease do not have time to act on the protein. Place the tissues in a ...

PIC Cross-linking and Immune Precipitation James FishburnHahn Lab March 2006 References Fishburn et.al.2005Molecular Cellvol.18 #3: Experimental Procedures pg.376 Immobilized Template assay (Hahn lab ...

Sample Buffer: 10 ml 0.06M Tris-HCl pH 6.8 0.6 ml 1M Tris 6.8 ...

1.Grow 25mls yeast cells to 5x10E6. 2.Sequentially spin down cells in a 15 ml polypro tube. 3.Wash cells with 1ml ice cold ddH2Otransferrring to an eppy tube. 4.Recon.cells in 1 ml ice cold ddH2O cont ...

HelveticaGenevaSwissSunSans-Regular" size="2"Linda Warfield Hahn Lab 2003 HelveticaGenevaSwissSunSans-Regular" size="2"Volumes given are for 2L of cells for each subunit (Toa1 and Toa2). Toa1 a ...

Purpose and backgrounds About nuclide... 35-S Decay: (b-ray0.167 MeV) Half life: 87.5 days Thick acrylic shield can block most of the emission.Because of the low energyit is impossible to detect spill ...

HIS-tagged protein purification John Mundy Institute of Molecular Biology Copenhagen Denmark http://www.arabidopsis.org/info/Protocols_Mundy2.jsp#his 1 liter cell prep 1) grow 20ml ...

Hancock Laboratory Methods.Department of Microbiology and Immunology University of British ColumbiaBritish ColumbiaCanada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=28 MATERIALS: Running buff ...

点击浏览该文件 ...

Polyacrylamide gel electrophoresis is a widely used technique to separate proteins from biological samples.Moreoverthe development of two-dimensional (2D)gel electrophoresis has provided a tool for di ...

Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer Steve Hahn Protein Dilution Buffer 5ml 20 mM Tris pH7.9 100 microliters 1M Tris 7.9 150 mM KCl 0.75 ml 1 M KCl 1 mM DTT 50 microliter ...

Hahn Lab 1.In microfuge tubesspin down 0.1 ml of uninduced cells grown to near saturation or 0.15 ml of IPTG induced cells.Remove YT (or LB)media with a pipetman or drawn out pasteur pipette and freez ...

Cell Lysis/Western/IP Steven FinkbeinerDepartments of Neurology and PhysiologyUCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Cell_Lysis.shtml compiled by 6/95 by AJS from AB)Fresh 1 ...

1.Crosslink protein DNA complexes in vivo Grow 50ml yeast culture to OD600 0.5-1.0.In fume hoodadd 1.4ml 37% fomaldehyde solution to a 50ml conical tube.Fill tube with culture to just below the 50ml l ...

Preparation of protein extracts for western blot 1.Grow cells to mid-log (OD600 less or equal to 1.0). 2.Harvest about 5 OD's of cells in a 13X100 glass dispo tube (Use new tubes.Can be up to about 6 ...

1. After electrophoresis of protein gel transfer gel to round staining tray. Add ~200 ml protein gel stain. 2. Microwave for ~45 sec until the s ...

高分子量标准参照 中分子量标准参物 &n ...

notes Written (2004-04-16)by Jonathan Sheehan Adapted from Pace and Sholtz1 Purpose When you really need to know the concentration of your urea or guanidinium HCl solutionsuse this method.Especially ...

This extraction procedure was developed by the Simmlaboratory and described in Rudolph et al.1999(Anal Biochem 26966-71).It’s a great way to make very concentrated extracts for Western blottinga ...