TE
1x; pH7.4; 7.6; 8.0; (store at 4o
C).
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
10ml
|
50ml
|
400ml
|
|
| Tris Cl
|
10mM
|
1M
|
10µl
|
50µl
|
100µl
|
500µl
|
4ml
|
|
| EDTA
|
1mM
|
0.5M
|
2µl
|
10µl
|
20µl
|
100µl
|
800µl
|
|
| H2
O
|
|
mQ
|
988µl
|
4.94ml
|
9.88ml
|
49.4ml
|
395ml
|
|
|
10x:
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
|
| Tris Cl
|
0.1M
|
1M
|
0.1ml
|
0.5ml
|
|
| EDTA
|
10mM
|
0.5M
|
20µl
|
0.1ml
|
|
| H2
O
|
mQ
|
|
880µl
|
4.4ml
|
|
|
- sterilize by autoclaving
STE
1x; (store at 4o
C).
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
10ml
|
50ml
|
|
| NaCl
|
0.1M
|
5M
|
20µl
|
100µl
|
200µl
|
1.0ml
|
|
| Tris Cl, pH8.0
|
10mM
|
1M
|
10µl
|
50µl
|
100µl
|
0.5ml
|
|
| EDTA, pH8.0
|
1mM
|
0.5M
|
2µl
|
10µl
|
20µl
|
100µl
|
|
| H2
O
|
|
mQ
|
0.968ml
|
4.84ml
|
9.68ml
|
48.4ml
|
|
|
10x:
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
|
| NaCl
|
1.0M
|
5M
|
0.2ml
|
1.0ml
|
|
| Tris Cl
|
0.1M
|
1M
|
0.1ml
|
0.5ml
|
|
| EDTA
|
10mM
|
0.5mM
|
20µl
|
0.1ml
|
|
| H2
O
|
mQ
|
|
680µl
|
3.4ml
|
|
|
- sterilize by autoclaving
SSC
20x; pH 7.0; (store at NT).
|
|
Conc.
|
Stock
|
0.5L
|
1.5L
|
2L
|
|
| NaCl
|
3M
|
58.44g/M
|
87.66g
|
263.0g
|
350.6g
|
|
| Na3
C6
H5
O7
x 5.5H2
O
|
0.3M
|
357.1g/M
|
53.57g
|
160.7g
|
214.3g
|
|
| H2
O
|
|
mQ
|
440.3ml
|
1.32L
|
1.76L
|
|
|
p = 1.103
TAE
1x pH = 7.6:
Tris-acetate 40mM
EDTA 1mM
store the main stock at 4o
C, working quantity (~100ml) at NT
|
|
Conc.
|
Stock
|
100ml
|
150ml
|
200ml
|
250ml
|
|
| Tris-base
|
2M
|
121.1g/M
|
24.22g
|
36.33g
|
48.44g
|
60.55g
|
|
| EDTA
|
0.05M
|
372.3g/M
|
1.862g
|
2.792g
|
3.723g
|
4.654g
|
|
| Acetic acid
|
~1.56M
|
17.4M
|
~8.96ml
~9.40g
|
~13.44ml
~14.10g
|
~17.92ml
~18.80g
|
~22.40ml
~23.50g
|
~
~
|
| H2
O
|
|
mQ
|
~73.30g
|
~109.95g
|
~146.60g
|
~183.25g
|
~
|
|
p(50x)=1.0878
it is convenient to solubilize Tris and EDTA first then adjust pH by acetic acid.
TBE
10x, pH ~8.3;(store at NT in clean bottle)
|
|
1x
|
10x
|
Stock
|
1L
|
|
| Tris base
|
89mM
|
0.89M
|
121.14g/M
|
107.8g
|
|
| Boric acid
|
89mM
|
0.89M
|
61.83g/M
|
55.03g
|
|
| EDTA, pH 8.0
|
2mM
|
20mM
|
500mM
|
40ml
43.84g
|
|
| H2
O
|
|
|
mQ
|
863.3ml
|
|
|
- 10x stock solution is stable at NT after 0.4µm filtration;
- for PAAG use as 1x, for agarose gel - as 0.5x;
- TBE is used in agarose gels for separation of small (< 200bp) fragments. Purification of fragments from TBE-based gels is more complicated than from TAE-based gels.
FGRB
50x (formaldehyde gel-running buffer), p=1.104g/ml (store at +4o
C):
|
|
Conc.
|
Stock
|
0.5L
|
1.0L
|
2.0L
|
|
| MOPS (pH7.0)
|
1.0M
|
209.27g/M
|
104.64g
|
209.27g
|
418.54g
|
|
| AcONax3H2
O
|
0.5M
|
136.08g/M
|
34.02g
|
68.04g
|
136.08g
|
|
| EDTAx2H2
O
|
50mM
|
372.3g/M
|
9.31g
|
18.62g
|
37.23g
|
|
| H2
O
|
|
mQ
|
404.0ml
|
808ml
|
1616ml
|
|
|
|
|
Conc.
|
Stock
|
0.5L
|
1.0L
|
2.0L
|
|
| MOPS (pH7.0)
|
1.0M
|
209.27g/M
|
104.64g
|
209.27g
|
418.54g
|
|
| AcONa anhydrous
|
0.5M
|
82.04g/M
|
20.51g
|
41.02g
|
82.04g
|
|
| EDTA x 2H2
O
|
50mM
|
372.3g/M
|
9.31g
|
18.62g
|
37.23g
|
|
| H2
O
|
|
mQ
|
417.5ml
|
835ml
|
1670ml
|
|
|
- adjust pH7.0 10N NaOH (per 500ml of buffer ~22ml), filter-sterilize through 0.22µm filter;
- solution becomes yellow during the storage on the light, but it has no effect on the solution quality.
GTGB
20x /Glycerol tolerant gel buffer/ (store at +4o
C):
|
|
Conc.
|
Stock
|
1L
|
1.5L
|
2.0L
|
2.5L
|
|
|
Tris base
|
1.78M
|
121.14g/M
|
216g
|
324g
|
432g
|
540g
|
|
|
Taurine
|
0.56M
|
125.15g/M
|
72g
|
108g
|
144g
|
180g
|
|
|
Na2
EDTAx2H2
O
|
10.7mM
|
372.3 g/M
|
4g
|
6g
|
8g
|
10g
|
|
|
H2
O
|
|
mQ
|
802ml
|
1203ml
|
1604ml
|
2005ml
|
|
|
p=1.094
- Taurine: C2
H7
NO3
S; Mw=125.15g/M.
TGB
Tris-Glycine buffer 5x, pH8.3, (store the main stock at +4o
C, working quantity at NT).
|
|
1x
|
Conc.
|
Stock
|
1L
|
1.5L
|
|
| Tris-base
|
25mM
|
125mM
|
121.1g/M
|
15.1g
|
22.71g
|
|
| Glycine
|
250mM
|
1.25M
|
75.05g/M
|
94g
|
141g
|
|
| SDS
|
0.1%
|
0.5%
|
10%
|
50ml
|
75ml
|
|
| H2
O
|
|
|
mQ
|
|
|
|
|
Loading buffer
10x, (store at NT).
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
10ml
|
25ml
|
|
| Xilene cyanol
|
0.1%
|
solid
|
1.0mg
|
5.0mg
|
10mg
|
25.0mg
|
|
| Bromph. blue
|
0.1%
|
solid
|
1.0mg
|
5.0mg
|
10mg
|
25.0mg
|
|
| SDS
|
0.5%
|
10%
|
50µl
|
250µl
|
500µl
|
1.25ml
|
|
| EDTA, pH8.0
|
0.1M
|
0.5M
|
0.2ml
|
1.0ml
|
2.0ml
|
5.0ml
|
|
| Glycerol
|
50%
|
100%
|
0.5ml
0.625g
|
2.5ml
3.125g
|
5.0ml
6.25g
|
12.5ml
15.63g
|
|
| H2
O
|
|
mQ
|
0.25ml
|
1.25ml
|
2.5ml
|
6.25ml
|
|
|
PEG-NaCl
(store at 4o
C).
|
|
Conc.
|
Stock
|
50ml
|
100ml
|
150ml
|
200ml
|
|
| NaCl
|
1.6M
|
58.44g/M
|
4.675g
|
9.35g
|
14.03g
|
18.70g
|
|
| PEG-6000
|
13%
|
solid
|
6.50g
|
13.00g
|
19.50g
|
26.00g
|
|
|
or:
|
|
Conc.
|
Stock
|
50ml
|
100ml
|
150ml
|
200ml
|
|
| NaCl
|
1.6M
|
5M
|
16ml
18.97g
|
32ml
37.95g
|
48ml
56.92g
|
64ml
75.90g
|
|
| PEG-6000
|
13%
|
40%
|
16.25ml
|
32.50ml
|
48.75ml
|
65.00ml
|
|
|
SM
(store at 4o
C).
|
|
Conc.
|
Stock
|
10ml
|
50ml
|
100ml
|
150ml
|
|
| NaCl
|
0.1M
|
5M
|
200µl
|
1.0ml
|
2.0ml
|
3.0ml
|
|
| MgSO4~undefined
|
10mM
|
1M
|
100µl
|
0.5ml
|
1.0ml
|
1.5ml
|
|
| Tris Cl, pH7.5
|
50mM
|
1M
|
0.5ml
|
2.5ml
|
5.0ml
|
7.5ml
|
|
|
|
0.01%
|
2%
|
50µl
|
0.25ml
|
0.5ml
|
0.75ml
|
|
| H2
O
|
|
mQ
|
9.15ml
|
45.75ml
|
91.5ml
|
137.25ml
|
|
|
- sterilize by autoclaving
STM
(store at 4o
C):
|
|
Conc.
|
Stock
|
50ml
|
|
| NaCl
|
10mM
|
5M
|
100µl
|
|
| Tris Cl pH8.0
|
50mM
|
1M
|
2.5ml
|
|
| MgCl2
|
10mM
|
1M
|
500µl
|
|
| H2
O
|
|
mQ
|
46.9ml
|
|
|
TM
(store at 4o
C):
|
|
Conc.
|
Stock
|
50ml
|
|
| Tris Cl pH8.0
|
50mM
|
1M
|
2.5ml
|
|
| MgCl2
|
10mM
|
1M
|
500µl
|
|
| H2
O
|
|
mQ
|
47.0ml
|
|
|
Denaturation buffer
(store at NT).
|
|
Conc.
|
Stock
|
100ml
|
150ml
|
300ml
|
500ml
|
750ml
|
|
| NaCl
|
1.5M
|
58.44g/M
|
8.766g
|
13.15g
|
26.30g
|
43.83g
|
65.75g
|
|
| NaOH
|
0.5M
|
10M
|
5ml
6.65g
|
7.5ml
9.97g
|
15ml
19.94g
|
25ml
33.23g
|
37.5ml
49.84g
|
|
|
|
|
40g/M
|
2g
|
3g
|
6g
|
10g
|
15g
|
|
|
Neutralization buffer
(store at NT).
|
|
Conc.
|
Stock
|
150ml
|
300ml
|
500ml
|
750ml
|
|
| Tris Cl, pH7.4
|
1M
|
121.1g/M
|
18.15g
|
36.3g
|
60.5g
|
90.75g
|
|
| NaCl
|
1.5M
|
58.44g/M
|
13.15g
|
26.30g
|
43.83g
|
65.75g
|
|
| HCl
|
~0.81M
|
11.6M/L
|
~10.5ml
|
~21ml
|
~35ml
|
~52.5ml
|
|
| EDTA (optional)
|
1mM
|
0.5M
|
0.3ml
|
0.6ml
|
1ml
|
1.5ml
|
|
|
Developer Rentgen-2
(store at NT in the dark).
|
|
500ml
|
800ml
|
1l
|
|
|
|
1.1g
|
1.76g
|
2.2g
|
|
| Na2
SO3
anhidr.
|
36.0g
|
57.6g
|
72.0g
|
|
| Hydroquinone
|
4.4g
|
7.04g
|
8.8g
|
|
| Na2
CO3
|
24.0g
|
38.4g
|
48.0g
|
|
| KBr
|
2.0g
|
3.2g
|
4.0g
|
|
| H2
O
|
490ml
|
784ml
|
980ml
|
|
|
solubilize in the presented order , p=1.115g/ml.
Fixer BKF-2
(store at NT).
|
|
500ml
|
800ml
|
1l
|
|
| Na2
S2
O3
x 5H2
O
|
130g
|
208g
|
260g
|
|
| NH4
Cl
|
25g
|
40g
|
50g
|
|
| Sodium pyrosulfite
|
8.5g
|
13.6g
|
17g
|
|
| H2
O
|
398ml
|
636.8ml
|
796ml
|
|
|
solubilize in the presented order, p=1.123g/ml.
Scintillation solution
(store at NT in the dark):
|
|
0.5L
|
1L
|
|
| PPO
|
2.50g
|
5.00g
|
|
| POPOP
|
0.05g
|
0.10g
|
|
| Toluene
|
|
|
|
|
Plasmid solution I
(store at +4o
C)
|
|
Conc.
|
Stock
|
50ml
|
100ml
|
150ml
|
200ml
|
|
| Glucose
|
50mM
|
2M
|
1.25ml
1.41g
|
2.5ml
2.825g
|
3.75ml
4.24g
|
5.0ml
5.56g
|
|
| Tris Cl, pH 8.0
|
25mM
|
1.0 M
|
1.25ml
|
2.5ml
|
3.75ml
|
5.0ml
|
|
| EDTA
|
10mM
|
0.5 M
|
1.0ml
|
2.0ml
|
3.0ml
|
4.0ml
|
|
| H2
O
|
|
mQ
|
46.5ml
|
93ml
|
139.5ml
|
186ml
|
|
|
Plasmid solution II
|
|
Conc.
|
Stock
|
0.5ml
|
1ml
|
2.5ml
|
5.0ml
|
6.5ml
|
8.5ml
|
|
| NaOH
|
0.2N
|
1N
|
0.1ml
|
0.2ml
|
0.5ml
|
1.0ml
|
1.3ml
|
1.7ml
|
|
| SDS
|
1%
|
10%
|
0.05ml
|
0.1ml
|
0.25ml
|
0.5ml
|
0.65ml
|
0.85ml
|
|
| H2
O
|
|
mQ
|
0.350ml
|
0.7ml
|
1.75ml
|
3.5ml
|
4.55ml
|
5.95ml
|
|
|
Oligonucleotide gel staining mix
(store at NT).
|
|
Conc.
|
Stock
|
100ml
|
200ml
|
300ml
|
500ml
|
|
| AcONa(5.2)
|
0.5M
|
3M
|
17ml
|
33ml
|
50.0ml
|
83ml
|
|
| Met. blue
|
0.04%
|
0.2%
|
20ml
|
40ml
|
60ml
|
100ml
|
|
| H2
O
|
|
mQ
|
73ml
|
127ml
|
190ml
|
317ml
|
|
|
Mg2+
2M
(store at NT).
|
|
Conc.
|
Stock
|
200ml
|
|
| MgCl2
x6H2
O
|
1M
|
203.3g/M
|
40.66g
|
|
| MgSO4
x7H2
O
|
1M
|
246.48g/M
|
49.30g
|
|
| H2
O
|
|
mQ
|
144.6g
|
|
|
p=1.173g/ml.
Hybridization buffer HSB (f+
)
high SDS hybridization buffer (store the main stock at 4o
C, working - NT)
|
|
Conc.
|
Stock
|
500ml
|
|
| SDS
|
7%
|
solid
|
35g
|
|
| Formamid
|
50%
|
100%
|
250ml
283.3g
|
|
| SSC
|
5x
|
20x
|
125ml
145.3g
|
|
| Bloc. Reagent
|
2%
|
solid
|
10g
|
|
| NaP (pH 7.0)
|
50mM
|
1M
|
25ml
27.6g
|
|
| N-Lauroylsarcosine Na
|
0.1%
|
10%
|
5ml
5.1g
|
|
| Salm.Sperm DNA
|
50µg/ml
|
10µg/µl
|
2.5ml
|
|
| H2
O
|
|
mQ
|
49ml
|
|
|
p=1.111
- Blocking Reagent - is the powder from "Roche Diagnostics GmBH; former Boehringer Mannheim" #1096176. Instead of 2% Blocking Reagent one may use 10x Denhardt or 2% non-fat dry milk.
- it is possible to dissolve the Blocking Reagent only in high strength buffer (in this particular case in H2
O+SSC+NaP).
- Salmon sperm DNA should be shredded and denaturated.
Restriction buffers 10x
А.
|
|
Conc.
|
Stock
|
100µl
|
1ml
|
|
| Tris Ac (pH7.9 37o
C)
|
33mM
|
1M
|
33µl
|
330µl
|
|
| MgAc
|
10mM
|
1M
|
10µl
|
100µl
|
|
| KAc
|
66mM
|
5M
|
13.2µl
|
132µl
|
|
| DTT
|
0.5mM
|
1M
|
0.5µl
|
5µl
|
|
| H2
O
|
|
mQ
|
43.3µl
|
433µl
|
|
|
B.
|
|
Conc.
|
Stock
|
100µl
|
1ml
|
|
| Tris Cl (pH 8.0 37o
C))
|
10mM
|
1M
|
10µl
|
100µl
|
|
| MgCl2
|
5mM
|
1M
|
5µl
|
50µl
|
|
| NaCl
|
100mM
|
5M
|
20µl
|
200µl
|
|
| b-Me
|
1mM
|
1M
|
1µl
|
10µl
|
|
| H2
O
|
|
mQ
|
64µl
|
640µl
|
|
|
L.
|
|
Conc.
|
Stock
|
100µl
|
1ml
|
|
| Tris Cl (pH 7.5 37o
C)
|
10mM
|
1M
|
10µl
|
100µl
|
|
| MgCl2
|
10mM
|
1M
|
10µl
|
100µl
|
|
| DTT
|
1mM
|
1M
|
1µl
|
10µl
|
|
| H2
O
|
|
mQ
|
79µl
|
790µl
|
|
|
M.
|
|
Conc.
|
Stock
|
100µl
|
1ml
|
|
| Tris Cl (pH7.5 37o
C)
|
10mM
|
1M
|
10µl
|
100µl
|
|
| NaCl
|
50mM
|
5M
|
10µl
|
100µl
|
|
| MgCl2
|
10mM
|
1M
|
10µl
|
100µl
|
|
| DTT
|
1mM
|
1M
|
1µl
|
10µl
|
|
| H2
O
|
|
mQ
|
69µl
|
690µl
|
|
|
H.
|
|
Conc.
|
Stock
|
100µl
|
1ml
|
|
| Tris Cl (pH7.5 37o
C)
|
50mM
|
1M
|
50µl
|
50µl
|
|
| MgCl2
|
10mM
|
1M
|
10µl
|
100µl
|
|
| NaCl
|
100mM
|
5M
|
20µl
|
200µl
|
|
| DTT
|
1mM
|
1M
|
1µl
|
10µl
|
|
| H2
0
|
|
mQ
|
19µl
|
190µl
|
|
|
Denhardt solution 50x
(store at -20o
C)
|
|
Conc.
|
Stock
|
50ml
|
300ml
|
1L
|
|
| Ficoll 400
|
1%(w/v)
|
solid
|
0.5g
|
3.0g
|
10g
|
|
| Polyvinilpyrolidone
|
1%(w/v)
|
solid
|
0.5g
|
3.0g
|
10g
|
|
| BSA (fr V)
|
1%(w/v)
|
solid
|
0.5g
|
3.0g
|
10g
|
|
| H2
O
|
|
mQ
|
46.7ml
|
292.2ml
|
974ml
|
|
|
p=1.004
- the solution should be filtered;
- the solution could be prepared as 100x stock.
PBS (recipe 1)
pH(1x)=7.4, (store at NT or at 4o
C):
|
|
1x
|
Conc.
|
Stock
|
100ml
|
500ml
|
|
| KH2
PO4
|
1.7mM
|
17mM
|
136.1g/M
|
0.231g
|
1.157g
|
|
| Na2
HPO4
|
5.2mM
|
52mM
|
142g/M
|
0.738g
|
3.692g
|
|
| NaCl
|
150mM
|
1.5M
|
58.44g/M
|
8.77g
|
43.83g
|
|
| H2
O
|
|
mQ
|
|
|
|
|
|
|
|
1x
|
Conc.
|
Stock
|
100ml
|
500ml
|
|
| KH2
PO4
|
1.7mM
|
17mM
|
136.1g/M
|
0.231g
|
1.157g
|
|
| Na2
HPO4
x7H2
O
|
5.2mM
|
52mM
|
268.1g/M
|
1.394g
|
6.971g
|
|
| NaCl
|
150mM
|
1.5M
|
58.44g/M
|
8.77g
|
43.83g
|
|
| H2
O
|
|
mQ
|
|
|
|
|
|
PBS (recipe 2) 10x
pH(1x)=7.3; (store at NT or at 4o
C)
|
|
1x
|
10x
|
Stock
|
100ml
|
500ml
|
|
| KH2
PO4
|
1.47mM
|
14.7mM
|
136.1g/M
|
0.2g
|
1.0g
|
|
| Na2
HPO4
x7H2
O
|
4.29mM
|
42.9mM
|
268.1g/M
|
1.15g
|
5.75g
|
|
| NaCl
|
137mM
|
1.37M
|
58.44g/M
|
8.0g
|
40.0g
|
|
| KCl
|
2.68mM
|
26.8mM
|
74.56g/M
|
0.2g
|
1.0g
|
|
| H2
O
|
|
|
mQ
|
|
|
|
|
PBS (recipe 3) 10x
pH(1x)=7.3; (store at NT or at 4o
C)
|
|
1x
|
10x
|
Stock
|
100ml
|
500ml
|
|
| KH2
PO4
|
2mM
|
20mM
|
136.1g/M
|
0.272g
|
1.36g
|
|
| Na2
HPO4
x7H2
O
|
10mM
|
100mM
|
268.1g/M
|
2.68g
|
13.41g
|
|
| NaCl
|
137mM
|
1.37M
|
58.44g/M
|
8.0g
|
40.0g
|
|
| KCl
|
2.7mM
|
27mM
|
74.56g/M
|
0.20g
|
1.01g
|
|
| H2
O
|
|
|
mQ
|
|
|
|
|
- sterilize by autoclaving;
- pH is concentration dependent; therefore check pH for diluted 1x buffer only;
- pH of 10x should be ~6.8 (adjust by NaOH if necessary).
SSPE 20x
pH 7.4
|
|
Conc.
|
Stock
|
1L
|
|
| NaCl
|
3M
|
58.44g/M
|
175g
|
|
| NaH2
PO4
xH2
O
|
200mM
|
137.99g/M
|
27.6g
|
|
| EDTA
|
20mM
|
372.24g/M
|
7.4g
|
|
| H2
O
|
|
mQ
|
926ml
|
|
|
- adjust pH by 10N NaOH.
RNase A (DNase free)
- solubilize 10mg/ml in 1-50mM AcONa (pH4.5);
- incubate 15' in boiling water bath, then cool slowly to NT;
- store at -20o
C, aliquote in use may be stored at 4o
C
RNAse storage buffer
(store at -20o
C).
|
|
Conc.
|
Stock
|
1ml
|
5ml
|
10ml
|
|
| Tris Cl, pH7.6
|
10mM
|
1M
|
10µl
|
50µl
|
100µl
|
|
| Glycerol
|
50%
|
100%
|
0.5ml
0.630g
|
2.5ml
3.152g
|
5.0ml
6.305g
|
|
| H2
O
|
|
mQ
|
0.486g
|
2.43g
|
4.86g
|
|
|
Proteinase K
(store at +4o
C, long-term storage at �20o
C):
20mg/ml solution in 10mM Tris Cl, 1.0mM CaCl2
, 30% glycerol.
EDTA/glycogen solution
20x (store at -20o
C):
|
|
Conc.
|
Stock
|
1.0ml
|
|
| EDTA
|
0.2M
|
0.5M
|
400µl
|
|
| glycogen
|
1mg/ml
|
20mg/ml
|
50µl
|
|
| H2
O
|
|
mQ
|
550µl
|
|
|
DNase RQ1 buffer
10x (store at -20o
C):
|
|
Conc.
|
Stock
|
1ml
|
|
| Tris Cl (pH8.025
o
C
)
|
0.4M
|
1M
|
400µl
|
|
| NaCl
|
0.1M
|
5M
|
20µl
|
|
| MgCl2
|
60mM
|
1M
|
60µl
|
|
| CaCl2
|
0.1M
|
1M
|
100µl
|
|
| H2
0
|
|
mQ
|
420µl
|
|
|
DNAse storage buffer
(store at -20o
C).
|
|
Conc.
|
Stock
|
1ml
|
1.5ml
|
2.5ml
|
5ml
|
10ml
|
|
| Tris Cl, pH 7.0
|
20mM
|
1M
|
20µl
|
30µl
|
50µl
|
100µl
|
200µl
|
|
| NaCl
|
50mM
|
5M
|
10µl
|
15µl
|
25µl
|
50µl
|
100µl
|
|
| DTT
|
1mM
|
1M
|
1µl
|
1.5µl
|
2.5µl
|
5.0µl
|
10µl
|
|
| Glycerol
|
50%
|
100%
|
0.5ml
0.630g
|
0.75ml
0.9g
|
1.25ml
1.58g
|
2.5ml
3.15g
|
5.0ml
6.31g
|
|
| H2
O
|
|
mQ
|
0.485ml
|
0.73ml
|
1.21ml
|
2.42ml
|
4.85ml
|
|
|
