常用试剂配制－2

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Acetone / Formaldehyde Fixative

Acrylamide Gel, denaturing

Acrylamide Gel, nondenaturing

Alkaline Phosphatase Buffer 1 (Glycine Buffer, 0.1 M, pH 10.4)

Alkaline Phosphatase Buffer 2 (Tris-HCl, 100 mM, pH 9.5)

Alkaline Phospatase Stain (NBT + BCIP)

Amido Black Destain

Ammonium Bicarbonate Buffer, 100x

Annealing Buffer

Antibiotics for Microbiology

Aprotinin Stock Solution

BLOTTO 1 x

Bouins Fixative

Butanol, water saturated

Cadmium Chloride Stock Solution

Calcium Chloride for Competent Cells

Calcium Chloride Stock Solution

CIP Buffer (10x)

Coomassie Destain

Coomassie Stain

Crystal Violet Stock Solution

Cytosol Preparation Buffer

DEAE DNA Elution Buffer I

DEAE DNA Elution Buffer II

DEAE Membrane Wash Buffer

Denhardt's, 50 x

DMEM/MCDB-302

DMEM

DTT Stock Solution

EDTA Stock Solution, 0.5 M

EMSA Binding Buffer

Fischer's Medium (for Nb2 cells)

Glycerol Stock Solution, 80%

HBS (HEPES buffered saline)

HBSS (Hanks Balanced Salt Solution)

HBHA (Hanks Balanced Salt Solution with BSA, HEPES, NaN₃ )

HeBS (Hepes buffered saline) buffer for electroporation

HEPES Stock Solution

IL-2 Stock Solution

Immunoprecipitation buffer (cell lysis buffer 2)

Immunoprecipitation dilution buffer

Immunoprecipitation wash buffer

IPTG Stock

KCl Stock Solution, 2 M

LB Agar

LB Medium

Lower Tris Buffer, 4x

Magnesium Chloride Stock Solution

MEM

NaCl Stock, 5 M

NaOH Stock, 10 N

NCTC (medium for RCHO-1 cells)

NZY broth

NZY plates

PBS / EDTA solution

PBS Stock Solution, 20x

PIPES Stock Solution

PMSF Stock 0.1 M

R10 (medium for KY1 and KY2 cells)

RIPA buffer (cell lysis buffer 1)

RNK (medium for RNK-16 cells)

RPMI 1640 (medium for YAC-1 cells)

SDS-PAGE, 4 x Running Buffer

SDS-PAGE, 4 x Sample Buffer

SDS Stock Solution, 10%, 20%

SM buffer

SOB-medium

SOC-medium

Sodium Acetate, 3 M

SSC, 20 x

SSPE, 20 x

Stacking Gel

STE, 1 X (for Nuc Trap)

STE for Genomic DNA Isolation

TAE Buffer Stock Solution, 50 x

TBE Stock Solution, 5 x

TBS/TBST buffer, 10x

TDMN Buffer

TE Buffer

Top Agar

Transfer Buffer (for Western Blotting)

Tris Stock Solution, 1 M

Upper Tris Buffer, 4x

Zinc Chloride Stock Solution

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