PCR and multiplex PCR guide

互联网2013-09-06

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<center> <h1> PCR and multiplex PCR guide </h1> </center> <center> <hr /> Table below lists the parameters influencing the PCR reaction and indicates some PCR and multiplex PCR applications. Clicking on an item in the table, will take you to a detailed page, describing that particular issue. <table> <tbody> <tr> <td> Topics for PCR optimization </td> </tr> <tr> <td> 0. Standards : examples of multiplex reactions used in all experiments </td> </tr> <tr> <td> 1 </td> <td> Generalities Components, PCR cycle, vials </td> <td> 9 </td> <td> Annealing time and temperature </td> </tr> <tr> <td> 2 </td> <td> Choosing PCR primers How to design the primers </td> <td> 10 </td> <td> Extension time and temperature </td> </tr> <tr> <td> 3 </td> <td> Reaction volumes Do they influence the results? </td> <td> 11 </td> <td> DNA template concentration </td> </tr> <tr> <td> 4 </td> <td> Number of PCR products Multiplexing. How ? </td> <td> 12 </td> <td> Taq polymerase(s) Comparisons and concentration </td> </tr> <tr> <td> 5 </td> <td> Primer amount How much primer? </td> <td> 13 </td> <td> dNTP concentration Interaction with magnesium </td> </tr> <tr> <td> 6 </td> <td> PCR buffers Comparisons and concentration </td> <td> 14 </td> <td> MgCl2 concentration Interaction with dNTP and salt (PCR buffer) </td> </tr> <tr> <td> 7 </td> <td> Salt (KCl) concentration Essential optimization factor </td> <td> 15 </td> <td> Gel electrophoresis Agarose and polyacrylamide (PAA) </td> </tr> <tr> <td> 8 </td> <td> Designing PCR programs Customize them for your purpose </td> <td> 16 </td> <td> Adjuvants in PCR DMSO, glycerol and BSA </td> </tr> <tr> <td> Some applications of PCR and multiplex PCR </td> </tr> <tr> <td> A </td> <td> Microdeletion screening </td> <td> D </td> <td> PCR labeling </td> </tr> <tr> <td> B </td> <td> LOH </td> <td> E </td> <td> InterAlu-PCR </td> </tr> <tr> <td> C </td> <td> Quantitative multiplex PCundefined </td> <td> F </td> <td> Degenerative priming PCR </td> </tr> </tbody> </table> </center>

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