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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
中乔新舟
- 库存:
1000
- 适应物种:
小鼠
- 规格:
T25
| 货号 |
CSP175 |
| 名称 |
小鼠 Direct PCR 基因分型快速检测试剂盒 |
| 描述 |
本试剂盒专为小鼠快速基因型鉴定而研制, 能够迅速从小鼠尾巴、耳朵或脚趾等组织中释 放足量的基因组DNA,消化时间仅需15分钟,无需抽提与纯化,可直接将消化产物作为 模板进行PCR扩增。试剂盒中配有高扩增兼容性的2xDet PCR SuperMix (Dye+) ,PCR 反应时只需加入引物和模板即可进行扩增,扩增产物可直接点样电泳。 PCR产物的3’端 带”A”,可进行TA克隆。 |
| 保存 |
-20°C保存6个月 |
| 供应限制 |
仅供科研使用 |
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文献和实验
Direct and Indirect In Situ PCR
In recent years, the development of in situ technologies has made good progress. In situ hybridization (ISH) has become an important tool and has enabled the pathologist to demonstrate infectious pathogens or mRNAs in tissue sections
for 60 minutes. 5) Add 100 μl FP3 Buffer and vortex to mix. 6) Store the extraction at 2-8°C. 2. PCR Protocol This protocol serves as a guideline for PCR amplification. Optimal reaction conditions, such as incubation times
Direct Sequencing of PCR Products
The development of the polymerase chain reaction (PCR) has allowed the rapid isolation of DNA sequences utilizing the hybridization of two oligonucleotide primers and subsequent amplification of the intervening sequences by Taq polymerase
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