Genomic DNA Quickprep for PCR
互联网
1440
macerate tissue in Eppendorf tube without butter at RT
add 400 m l extraction buffer
vortex for 4 sec
leave sample at RT until other samples are ready (> 1 h)
spin in microfuge for 1 min
transfer 300 m l of supernatant to different Eppendorf tube (prefilled with 300 m l isopropanole)
mix and leave at RT for 2 min
spin for 5 min
vacuum dry pellet and take up in 100 m l TE
use 1-2.5 m l for PCR
Remarks:
DNA is stable for one year at 4℃
Solutions:
Extraction buffer:
200 mM Tris-HCl pH 7.5
250 mM NaCl
25 mM EDTA
0.5% SDS
