Genomic DNA Quickprep for PCR

1. macerate tissue in Eppendorf tube without butter at RT
2. add 400 m l extraction buffer
3. vortex for 4 sec
4. leave sample at RT until other samples are ready (> 1 h)
5. spin in microfuge for 1 min
6. transfer 300 m l of supernatant to different Eppendorf tube (prefilled with 300 m l isopropanole)
7. mix and leave at RT for 2 min
8. spin for 5 min
9. vacuum dry pellet and take up in 100 m l TE
10. use 1-2.5 m l for PCR

Remarks:

DNA is stable for one year at 4°C