Trichovirus Isolation and RNA Extraction

The genus Trichovirus, a newly established plant virus genus (1 ), contains five viral species (including three tentative members), with similar biological, morphological, physicochemical, and ultrastructural properties. Apple chlorotic leaf spot virus (ACLSV) and potato virus T (PVT) are definitive members of the genus (2 ,3 ), whereas heracleum latent virus (HLV) (4 ), grapevine virus A (GVA), and grapevine virus B (GVB) (5 ,6 ) are regarded as putative members.