TAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the Heart
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TAT protein transduction is a novel method of delivering biologically active proteins into cells and tissues through the fusion of a protein transduction domain to the protein of interest. The present chapter outlines the methodology pertaining to the preparation of TAT-fusion proteins and how to efficiently transduce these proteins into cultured cells or isolated rat hearts perfused in Langendorff mode.
