常用的β-actin 引物序列

human actin f ctc cat cct ggc ctc gct gt
human actin r gct gtc acc ttc acc gtt cc
product size:268
rabbit actin r agt gcg acg tgg aca tcc g
rabbit actin f tgg ctc taa cag tcc gcc tag
product size:295
mouse actin r cgt tga cat ccg taa aga cc
mouse actin f aac agt ccg cct aga agc ac
product size:281
rat actin f TCAGGTCATCACTATCGGCAAT
rat actin r AAAGAAAGGGTGTAAAACGCA
product size:432
human actin r gag cta cga gct gcc tga cg
human actin f cct aga agc att tgc ggt gg
product size:416
mouse actin f tca tca cta ttg gca acg agc
mouse actin r aac agt ccg cct aga agc ac
product size:399
rat actin f CCCATCTATGAGGGTTACGC
rat actin r TTTAATGTCACGCACGATTTC
product size:150
rabbit actin f tct tcc agc cct cct tcc tg
rabbit actin r cgt ttc tgc gcc gtt agg t
product size:409
内参基因名称 引物 引物最佳退火 扩增
基因库序列号 引物名称 序列 位置 Tm 温度C 长度

Human actin beta F305 ctgggacgacatggagaaaa 305-324 52.3
BC002409 R868 aaggaaggctggaagagtgc 868-849 52.6 59.4 564
F1379 agcgagcatcccccaaagtt 1379-1398 57.3
R1663 gggcacgaaggctcatcatt 1663-1644 56.3 54 285

Rat actin beta F18 cacccgcgagtacaaccttc 18-37 54.5
NM_031144 R224 cccatacccaccatcacacc 224-205 54.4 60.4 207
F694 gagagggaaatcgtgcgtgac 694-714 54
R1146 catctgctggaaggtggaca 1146-1127 53.2 57.1 452

Mouse actin beta F91 atatcgctgcgctggtcgtc 91-110 57.5
NM_007393 R607 aggatggcgtgagggagagc 607-588 57.8 60.4 517
F1566 gtccctcaccctcccaaaag 1566-1585 54.5
F1831 gctgcctcaacacctcaaccc 1831-1811 54.4 55.7 266

human GAPDH F369 agaaggctggggctcatttg 369-388 55.6
BC004109 R626 aggggccatccacagtcttc 626-607 55.1 57.5 258
F66 aggtcggagtcaacggatttg 66-86 52.5
R597 gtgatggcatggactgtggt 597-578 52.6 57 532

Rat GAPDH F50 cagtgccagcctcgtctcat 50-69 55
BC059110 R644 aggggccatccacagtcttc 644-625 55.1 58.3 595
F1029 acagcaacagggtggtggac 1029-1048 54.4
R1280 tttgagggtgcagcgaactt 1280-1261 54.8 57.7 252

Mouse GAPDH F306 aggccggtgctgagtatgtc 306-325 54
M32599 R835 tgcctgcttcaccaccttct 835-816 54.6 58.7 530
F49 ggtgaaggtcggtgtgaacg 49-68 55.1
R281 ctcgctcctggaagatggtg 281-262 54.5 56.8 233

Rat 18S rRNA F1865 tgcggaaggatcattaacgga 1865-1886 58.6
M11188 R2164 agtaggagaggagcgagcgacc 2164-2143 59 59.3 300

human 18S rRNA F1565 cagccacccgagattgagca 1565-1584 58.3
M10098 R1816 tagtagcgacgggcggtgtg 1816-1797 58.3 59 253

mouse 18S rRNA F56 aggggagagcgggtaagaga 56-75 54.4
K013364 R296 ggacaggactaggcggaaca 296-277 54 62 241