• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Combination of Chemical and Enzymatic RNA Synthesis

        互联网

        420
        The potential of standard in vitro transcription reactions can be dramatically expanded, if chemically synthesized low-mol-wt compounds are used as building blocks in combination with standard nucleotide 5′ triphosphates (NTPs). Short oligonucleotides that terminate in guanosine effectively compete with guanosine 5′ triphosphate (GTP) as starter building blocks, and they are incorporated at the 5′-end of transcripts. Applications include production of RNAs with “unfriendly 5′-ends” (they do not begin with G), variations of the 5′-sequence are possible with the same DNA template, site-specific insertion of nucleotide modifications, and addition of 5′-labels, such as fluorescein for detection or biotin for capture. Clearly, chemically synthesized, modified NTPs are inserted at internal sites. The combination with phosphorothioate linkages for detection has been developed into a powerful high-throughput method to study site-specific interference of modifications with RNA function.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序