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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2b
- 保存条件:
Store at -80°C.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
chemical compound
- 宿主:
Mouse
- 应用范围:
IHC, Dot Blot, ELISA, Indirect ELISA
- 靶点:
m7G
- 抗体英文名:
Chemical compound m7G Monoclonal antibody, PBS Only
- 抗体名:
Chemical compound m7G Monoclonal antibody, PBS Only
- 规格:
100ug
产品信息
68302-1-PBS targets m7G in IHC, Dot Blot, ELISA, Indirect ELISA applications and shows reactivity with chemical compound samples.
| 经测试应用 | IHC, Dot Blot, ELISA, Indirect ELISA |
| 经测试反应性 | chemical compound |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | m7G |
| 别名 | 7-Methylguanosine |
| 基因名称 | |
| Gene ID (NCBI) | |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS only, pH 7.3. |
| 储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
7-Methylguanosine (m7G) is a modified purine nucleoside. It is a methylated version of guanosine and when found in human urine, it may be a biomarker of some types of cancer. In the RNAs, 7-methylguanosine have been used to study and examine the reaction evolving methylguanosine. It also plays a role in mRNA as a blocking group at its 5´-end. The m7G modification actively participates in biological and pathological functions by affecting the metabolism of various RNA molecules, including messenger RNA, ribosomal RNA, microRNA, and transfer RNA. Increasing evidence indicates a critical role for m7G in human disease development, especially cancer, and aberrant m7G levels are closely associated with tumorigenesis and progression via regulation of the expression of multiple oncogenes and tumor suppressor genes.
Protocol for Dot Blot:
https://www.ptglab.com/protocol/68302-1-IgDotBlot.pdf
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文献和实验质沉淀,尤其是PI值在4.5附近的白蛋白,将这些杂蛋白用辛酸沉淀完毕后,再用法将抗体沉淀下来即可。 具体操作: (1)将腹水用0.06 M醋酸钠(pH4.0)按1:3稀释,在4 ℃条件下搅拌加入正辛酸,一般按每毫升未稀释的腹水加入40 ul正辛酸,然后4 ℃静置2 h; (2)4 ℃10000 g离心20min,可见辛酸因温度低而从系统中结晶析出漂浮在腹水上层,弃去此结晶以及底部沉淀,向上清中加入1/10体积的10×PBS(0.1M,pH 7.4),4 ℃条件下向其中加
Chemical Induction of Apoptosis
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一、材料及试剂 1、猪瘟单抗纯化抗原 2、兔抗猪IgG酶标抗体 3、猪瘟阳性血清 4、猪瘟阴性血清 1~4均由指定的生物制品所购买。 5、ELISA反应板 6、包被液 碳酸钠 1.50g 碳酸氢钠 2.93g H2O加至 1 000ml pH值9.6 7、PBS液 氯化钠 8.90g 磷酸二氢钾 0.20g 无水磷酸氢二钠 2.13g 加双馏水 1 000ml 8、PBS-吐温洗涤液 PBS液 1 000ml 犊牛血清 5ml 混合即可 9、底物
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