Quick Yeast DNA Prep: Isolation of Total DNA (genomic and plasmid)

互联网2013-09-06

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Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg.

Transfer culture to a small 13 x 100 glass tube. Spin down cells 2 min. in tabletop centrifuge. Pour off supernatant.

Wash cells with 5 ml H₂ O. Spin down cells 2 min. Pour off supernatant. Cell pellet can be stored at -20 deg.

Resuspend cells in 500 ul lysis buffer by vortexing.

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