Quick Yeast DNA Prep: Isolation of Total DNA (genomic and plasmid)

互联网2013-09-06

889

Grow a 5 ml YPD O/N culture inoculated with a single yeast colony at 30 deg.

Transfer culture to a small 13 x 100 glass tube. Spin down cells 2 min. in tabletop centrifuge. Pour off supernatant.

Wash cells with 5 ml H₂ O. Spin down cells 2 min. Pour off supernatant. Cell pellet can be stored at -20 deg.

Resuspend cells in 500 ul lysis buffer by vortexing.

相关产品推荐

mRNA纯化试剂盒|高效、灵敏、高性价比| mRNA Isolation Master Kit

￥2600

1 kb DNA分子量标准

￥100

土壤总磷酸盐含量检测试剂盒，用于对样本中Total Phosphorus含量检测，Soil Total Phosphate Assay Kit

￥598

An12g08930/An12g08930蛋白Recombinant A_s_p_ergillus niger A_s_p_ergillus niger contig An12c0300, genomic contig (An12g08930)重组蛋白/蛋白

￥2328

Prolyl Endopeptidase重组蛋白|Recombinant Mouse Prolyl endopeptidase / PREP Protein (His Tag)

￥2310

无忧采购轻松科研

提问

扫一扫

实验小助手

扫码领资料

反馈

TOP

打开小程序