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        请各位同道用blast帮我分析一下我的引物是否合适。急!!!先谢谢大家了

        丁香园论坛

        851
        AIB1-U primer (5'-ATA CTT GCT GGA TGG TGG ACT-3'; sense; positions 110–130; GenBank accession no. AF012108)
        AIB1-L primer (5'-TCC TTG CTC TTT TAT TTG ACG-3'; antisense; positions 458–438; GenBank accession no. AF012108);
        如上为我的目的基因上下游引物,请各位同道帮忙用软件及blast分析一下,本人非常焦急,因试验结果很不好,正在考虑是否更换引物!先谢谢大家了!
        如下为我的我的目的基因的基因组序列
        1 cggcggcggc tgcggcttag tcggtggcgg ccggcggcgg ctgcgggctg agcggcgagt
        61 ttccgattta aagctgagct gcgaggaaaa tggcggcggg aggatcaaaa tacttgctgg
        121 atggtggact cagagaccaa taaaaataaa ctgcttgaac atcctttgac tggttagcca
        181 gttgctgatg tatattcaag atgagtggat taggagaaaa cttggatcca ctggccagtg
        241 attcacgaaa acgcaaattg ccatgtgata ctccaggaca aggtcttacc tgcagtggtg
        301 aaaaacggag acgggagcag gaaagtaaat atattgaaga attggctgag ctgatatctg
        361 ccaatcttag tgatattgac aatttcaatg tcaaaccaga taaatgtgcg attttaaagg
        421 aaacagtaag acagatacgt caaataaaag agcaaggaaa aactatttcc aatgatgatg
        481 atgttcaaaa agccgatgta tcttctacag ggcagggagt tattgataaa gactccttag
        541 gaccgctttt acttcaggca ttggatggtt tcctatttgt ggtgaatcga gacggaaaca
        601 ttgtatttgt atcagaaaat gtcacacaat acctgcaata taagcaagag gacctggtta
        661 acacaagtgt ttacaatatc ttacatgaag aagacagaaa ggattttctt aagaatttac
        721 caaaatctac agttaatgga gtttcctgga caaatgagac ccaaagacaa aaaagccata
        781 catttaattg ccgtatgttg atgaaaacac cacatgatat tctggaagac ataaacgcca
        841 gtcctgaaat gcgccagaga tatgaaacaa tgcagtgctt tgccctgtct cagccacgag
        901 ctatgatgga ggaaggggaa gatttgcaat cttgtatgat ctgtgtggca cgccgcatta
        961 ctacaggaga aagaacattt ccatcaaacc ctgagagctt tattaccaga catgatcttt
        1021 caggaaaggt tgtcaatata gatacaaatt cactgagatc ctccatgagg cctggctttg
        1081 aagatataat ccgaaggtgt attcagagat tttttagtct aaatgatggg cagtcatggt
        1141 cccagaaacg tcactatcaa gaagcttatc ttaatggcca tgcagaaacc ccagtatatc
        1201 gattctcgtt ggctgatgga actatagtga ctgcacagac aaaaagcaaa ctcttccgaa
        1261 atcctgtaac aaatgatcga catggctttg tctcaaccca cttccttcag agagaacaga
        1321 atggatatag accaaaccca aatcctgttg gacaagggat tagaccacct atggctggat
        1381 gcaacagttc ggtaggcggc atgagtatgt cgccaaacca aggcttacag atgccgagca
        1441 gcagggccta tggcttggca gaccctagca ccacagggca gatgagtgga gctaggtatg
        1501 ggggttccag taacatagct tcattgaccc ctgggccagg catgcaatca ccatcttcct
        1561 accagaacaa caactatggg ctcaacatga gtagcccccc acatgggagt cctggtcttg
        1621 ccccaaacca gcagaatatc atgatttctc ctcgtaatcg tgggagtcca aagatagcct
        1681 cacatcagtt ttctcctgtt gcaggtgtgc actctcccat ggcatcttct ggcaatactg
        1741 ggaaccacag cttttccagc agctctctca gtgccctgca agccatcagt gaaggtgtgg
        1801 ggacttccct tttatctact ctgtcatcac caggccccaa attggataac tctcccaata
        1861 tgaatattac ccaaccaagt aaagtaagca atcaggattc caagagtcct ctgggctttt
        1921 attgcgacca aaatccagtg gagagttcaa tgtgtcagtc aaatagcaga gatcacctca
        1981 gtgacaaaga aagtaaggag agcagtgttg agggggcaga gaatcaaagg ggtcctttgg
        2041 aaagcaaagg tcataaaaaa ttactgcagt tacttacctg ttcttctgat gaccggggtc
        2101 attcctcctt gaccaactcc cccctagatt caagttgtaa agaatcttct gttagtgtca
        2161 ccagcccctc tggagtctcc tcctctacat ctggaggagt atcctctaca tccaatatgc
        2221 atgggtcact gttacaagag aagcaccgga ttttgcacaa gttgctgcag aatgggaatt
        2281 caccagctga ggtagccaag attactgcag aagccactgg gaaagacacc agcagtataa
        2341 cttcttgtgg ggacggaaat gttgtcaagc aggagcagct aagtcctaag aagaaggaga
        2401 ataatgcact tcttagatac ctgctggaca gggatgatcc tagtgatgca ctctctaaag
        2461 aactacagcc ccaagtggaa ggagtggata ataaaatgag tcagtgcacc agctccacca
        2521 ttcctagctc aagtcaagag aaagacccta aaattaagac agagacaagt gaagagggat
        2581 ctggagactt ggataatcta gatgctattc ttggtgatct gactagttct gacttttaca
        2641 ataattccat atcctcaaat ggtagtcatc tggggactaa gcaacaggtg tttcaaggaa
        2701 ctaattctct gggtttgaaa agttcacagt ctgtgcagtc tattcgtcct ccatataacc
        2761 gagcagtgtc tctggatagc cctgtttctg ttggctcaag tcctccagta aaaaatatca
        2821 gtgctttccc catgttacca aagcaaccca tgttgggtgg gaatccaaga atgatggata
        2881 gtcaggaaaa ttatggctca agtatgggtg ggccaaaccg aaatgtgact gtgactcaga
        2941 ctccttcctc aggagactgg ggcttaccaa actcaaaggc cggcagaatg gaacctatga
        3001 attcaaactc catgggaaga ccaggaggag attataatac ttctttaccc agacctgcac
        3061 tgggtggctc tattcccaca ttgcctcttc ggtctaatag cataccaggt gcgagaccag
        3121 tattgcaaca gcagcagcag atgcttcaaa tgaggcctgg tgaaatcccc atgggaatgg
        3181 gggctaatcc ctatggccaa gcagcagcat ctaaccaact gggttcctgg cccgatggca
        3241 tgttgtccat ggaacaagtt tctcatggca ctcaaaatag gcctcttctt aggaattccc
        3301 tggatgatct tgttgggcca ccttccaacc tggaaggcca gagtgacgaa agagcattat
        3361 tggaccagct gcacactctt ctcagcaaca cagatgccac aggcctggaa gaaattgaca
        3421 gagctttggg cattcctgaa cttgtcaatc agggacaggc attagagccc aaacaggatg
        3481 ctttccaagg ccaagaagca gcagtaatga tggatcagaa ggcaggatta tatggacaga
        3541 catacccagc acaggggcct ccaatgcaag gaggctttca tcttcaggga caatcaccat
        3601 cttttaactc tatgatgaat cagatgaacc agcaaggcaa ttttcctctc caaggaatgc
        3661 acccacgagc caacatcatg agaccccgga caaacacccc caagcaactt agaatgcagc
        3721 ttcagcagag gctgcagggc cagcagtttt tgaatcagag ccgacaggca cttgaattga
        3781 aaatggaaaa ccctactgct ggtggtgctg cggtgatgag gcctatgatg cagccccagc
        3841 agggttttct taatgctcaa atggtcgccc aacgcagcag agagctgcta agtcatcact
        3901 tccgacaaca gagggtggct atgatgatgc agcagcagca gcagcagcaa cagcagcagc
        3961 agcagcagca gcagcagcaa cagcaacagc aacagcaaca gcagcaacag cagcaaaccc
        4021 aggccttcag cccacctcct aatgtgactg cttcccccag catggatggg cttttggcag
        4081 gacccacaat gccacaagct cctccgcaac agtttccata tcaaccaaat tatggaatgg
        4141 gacaacaacc agatccagcc tttggtcgag tgtctagtcc tcccaatgca atgatgtcgt
        4201 caagaatggg tccctcccag aatcccatga tgcaacaccc gcaggctgca tccatctatc
        4261 agtcctcaga aatgaagggc tggccatcag gaaatttggc caggaacagc tccttttccc
        4321 agcagcagtt tgcccaccag gggaatcctg cagtgtatag tatggtgcac atgaatggca
        4381 gcagtggtca catgggacag atgaacatga accccatgcc catgtctggc atgcctatgg
        4441 gtcctgatca gaaatactgc tgacatctct gcaccaggac ctcttaagga aaccactgta
        4501 caaatgacac tgcactagga ttattgggaa ggaatcattg ttccaggcat ccatcttgga
        4561 agaaaggacc agctttgagc tccatcaagg gtattttaag tgatgtcatt tgagcaggac
        4621 tggattttaa gccgaagggc aatatctacg tgtttttccc ccctccttct gctgtgtatc
        4681 atggtgttca aaacagaaat gttttttggc attccacctc ctagggatat aattctggag
        4741 acatggagtg ttactgatca taaaactttt gtgtcacttt tttctgcctt gctagccaaa
        4801 atctcttaaa tacacgtagg tgggccagag aacattggaa gaatcaagag agattagaat
        4861 atctggtttc tctagttgca gtattggaca aagagcatag tcccagcctt caggtgtagt
        4921 agttctgtgt tgaccctttg tccagtggaa ttggtgattc tgaattgtcc tttactaatg
        4981 gtgttgagtt gctctgtccc tattatttgc cctaggcttt ctcctaatga aggttttcat
        5041 ttgccattca tgtcctgtaa tacttcacct ccaggaactg tcatggatgt ccaaatggct
        5101 ttgcagaaag gaaatgagat gacagtattt aatcgcagca gtagcaaact tttcacatgc
        5161 taatgtgcag ctgagtgcac tttatttaaa aagaatggat aaatgcaata ttcttgaggt
        5221 cttgagggaa tagtgaaaca cattcctggt ttttgcctac acttacgtgt tagacaagaa
        5281 ctatgatttt ttttttaaag tactggtgtc accctttgcc tatatggtag agcaataatg
        5341 ctttttaaaa ataaacttct gaaaacccaa ggccaggtac tgcattctga atcagaatct
        5401 cgcagtgttt ctgtgaatag atttttttgt aaatatgacc tttaagatat tgtattatgt
        5461 aaaatatgta tatacctttt tttgtaggtc acaacaactc atttttacag agtttgtgaa
        5521 gctaaatatt taacattgtt gatttcagta agctgtgtgg tgaggctacc agtggaagag
        5581 acatcccttg acttttgtgg cctgggggag gggtagtgct ccacagcttt tccttcccca
        5641 ccccccagcc ttagatgcct cgctcttttc aatctcttaa tctaaatgct ttttaaagag
        5701 attatttgtt tagatgtagg cattttaatt ttttaaaaat tcctctacca gaactaagca
        5761 ctttgttaat ttggggggaa agaatagata tggggaaata aacttaaaaa aaaatcagga
        5821 atttaaaaaa acgagcaatt tgaagagaat cttttggatt ttaagcagtc cgaaataata
        5881 gcaattcatg ggctgtgtgt gtgtgtgtat gtgtgtgtgt gtgtgtgtat gtttaattat
        5941 gttacctttt catccccttt aggagcgttt tcagattttg gttgctaaga cctgaatccc
        6001 atattgagat ctcgagtaga atccttggtg tggtttctgg tgtctgctca gctgtcccct
        6061 cattctacta atgtgatgct ttcattatgt ccctgtggat tagaatagtg tcagttattt
        6121 cttaagtaac tcagtaccca gaacagccag ttttactgtg attcagagcc acagtctaac
        6181 tgagcacctt ttaaacccct ccctcttctg ccccctacca cttttctgct gttgcctctc
        6241 tttgacacct gttttagtca gttgggagga agggaaaaat caagtttaat tccctttatc
        6301 tgggttaatt catttggttc aaatagttga cggaattggg tttctgaatg tctgtgaatt
        6361 tcagaggtct ctgctagcct tggtatcatt ttctagcaat aactgagagc cagttaattt
        6421 taagaatttc acacatttag ccaatctttc tagatgtctc tgaaggtaag atcatttaat
        6481 atctttgata tgcttacgag taagtgaatc ctgattattt ccagacccac caccagagtg
        6541 gatcttattt tcaaagcagt atagacaatt atgagtttgc cctctttccc ctaccaagtt
        6601 caaaatatat ctaagaaaga ttgtaaatcc gaaaacttcc attgtagtgg cctgtgcttt
        6661 tcagatagta tactctcctg tttggagaca gaggaagaac caggtcagtc tgtctctttt
        6721 tcagctcaat tgtatctgac ccttctttaa gttatgtgtg tggggagaaa tagaatggtg
        6781 ctcttatctt tcttgacttt aaaaaaatta ttaaaaacaa aaaaaaaaaa aaaaa
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