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        下面是我设计的引物的BLAST结果,请大虾帮我解释一下。谢谢

        丁香园论坛

        2708
        下面是我设计的引物的BLAST结果,请大虾帮我解释一下。我这对引物做PCR是否有问题?谢谢。Query=
        (40 letters)

        Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS,
        GSS,environmental samples or phase 0, 1 or 2 HTGS sequences)
        2,862,112 sequences; 12,768,194,998 total letters

        If you have any problems or questions with the results of this search please refer to the BLAST FAQs
        Taxonomy reports

        Distribution of 83 Blast Hits on the Query Sequence


        --------------------------------------------------------------------------------

        Score E
        Sequences producing significant alignments: (bits) Value

        gi|28315973|tpe|BN000065.1|HSA000065 TPA: Homo sapiens SMP1... 46 0.004
        gi|49256907|emb|AL928711.18| Human DNA sequence from clone ... 46 0.004
        gi|40733884|emb|BX855602.2| Human DNA sequence from clone X... 46 0.004
        gi|21750288|dbj|AK091825.1| Homo sapiens cDNA FLJ34506 fis,... 46 0.004
        gi|37665388|emb|BX649364.3| Human DNA sequence from clone R... 46 0.004
        gi|34494689|emb|BX640519.2| Human DNA sequence from clone R... 46 0.004
        gi|6002137|emb|AL031284.9|HS469D22 Human DNA sequence from ... 46 0.004
        gi|46389547|ref|NG_003255.1| Homo sapiens Rhesus blood grou... 46 0.004
        gi|2739326|emb|Z97333.1|HSRHCE567 Homo sapiens RHCE gene, (... 46 0.004
        gi|4150997|emb|Z97334.1|HSRHD567 Homo sapiens RHD gene, (pa... 46 0.004
        gi|9955424|dbj|AB035199.1| Homo sapiens RHCE gene, intron 6... 46 0.004
        gi|9955425|dbj|AB035200.1| Homo sapiens RHD gene, intron 6,... 46 0.004
        gi|20339039|emb|AL139426.16| Human DNA sequence from clone ... 46 0.004
        gi|26801335|gb|AC121585.3| Mus musculus BAC clone RP23-265M... 38 0.87
        gi|4176442|emb|AL022238.1|HS1042K10 Human DNA sequence from... 38 0.87
        gi|38565518|gb|AY449385.1| Homo sapiens rhesus blood group ... 36 3.4
        gi|38565516|gb|AY449384.1| Homo sapiens rhesus blood group ... 36 3.4
        gi|38565514|gb|AY449383.1| Homo sapiens rhesus blood group ... 36 3.4
        gi|38565512|gb|AY449382.1| Homo sapiens rhesus blood group ... 36 3.4
        gi|38565510|gb|AY449381.1| Homo sapiens rhesus blood group ... 36 3.4
        gi|20336224|ref|NM_016124.2| Homo sapiens Rhesus blood grou... 36 3.4
        gi|20336226|ref|NM_016225.2| Homo sapiens Rhesus blood grou... 36 3.4
        gi|55831592|gb|AC151778.1| Ornithorhynchus anatinus chromos... 36 3.4
        gi|53794104|gb|AY751496.1| Homo sapiens isolate Del9+170 Rh... 36 3.4
        gi|53794102|gb|AY751495.1| Homo sapiens isolate Del89+170 R... 36 3.4
        gi|53794100|gb|AY751494.1| Homo sapiens isolate Del9 Rhesus... 36 3.4
        gi|53794098|gb|AY751493.1| Homo sapiens isolate Del89 Rhesu... 36 3.4
        gi|53794096|gb|AY751492.1| Homo sapiens isolate Del79 Rhesu... 36 3.4
        gi|53794094|gb|AY751491.1| Homo sapiens isolate Del789 Rhes... 36 3.4
        gi|45379256|emb|BX784036.6| Zebrafish DNA sequence from clo... 36 3.4
        gi|21311250|gb|AF390114.1| Homo sapiens Rhesus blood group ... 36 3.4
        gi|56562202|emb|AJ867775.1| Homo sapiens partial RHCE gene ... 36 3.4
        gi|40714024|emb|BX248328.6| Zebrafish DNA sequence from clo... 36 3.4
        gi|56409833|emb|AJ867387.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|50871786|emb|AJ704215.2| Homo sapiens partial RHD gene f... 36 3.4
        gi|50657038|emb|AJ784312.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|50657036|emb|AJ784311.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|50657034|emb|AJ784310.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|50657032|emb|AJ784309.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|20503262|gb|AC116631.6| Homo sapiens BAC clone RP11-429B... 36 3.4
        gi|10800053|dbj|AB049754.1| Homo sapiens RHD mRNA for Rh bl... 36 3.4
        gi|38153716|emb|AJ580940.1|HSA580940 Homo sapiens partial R... 36 3.4
        gi|37650533|emb|AJ585040.1| Homo sapiens partial RHD gene f... 36 3.4
        gi|36046|emb|X63097.1|HSRHXIII H.sapiens mRNA for rhesus po... 36 3.4
        gi|21907993|dbj|AP004975.1| Lotus corniculatus var. japonic... 36 3.4
        gi|16040905|emb|AJ276015.1|HSA276015 Homo sapiens partial R... 36 3.4
        gi|7018341|emb|AJ287294.1|HSA287294 Homo sapiens partial RH... 36 3.4
        gi|10334531|emb|AJ299025.1|HSA299025 Homo sapiens partial R... 36 3.4
        gi|18093076|gb|AC097484.3| Homo sapiens BAC clone RP11-147K... 36 3.4
        gi|25573111|gb|AF510070.1| Homo sapiens Rhesus blood group ... 36 3.4
        gi|25573107|gb|AF510069.1| Homo sapiens Rhesus blood group ... 36 3.4
        gi|6164858|gb|AF187846.1|AF187846 Homo sapiens RhD type III... 36 3.4
        gi|5911384|gb|AF177938.1|AF177938 Homo sapiens Rh blood gro... 36 3.4
        gi|11128446|gb|AC084732.1|AC084732 Homo sapiens BAC clone R... 36 3.4
        gi|4104640|gb|AF037626.1|AF037626 Homo sapiens truncated Rh... 36 3.4
        gi|11878260|gb|AF312679.1|AF312679 Homo sapiens rhesus D ca... 36 3.4
        gi|999309|gb|S78509.1|S78509 RhD [human, D--phenotype, eryt... 36 3.4
        gi|693866|gb|S73913.1|S73913 Rh D=RhK562-II [human, erythro... 36 3.4
        gi|546725|gb|S70174.1|S70174 RhD=Rh blood group system D-an... 36 3.4
        gi|299051|gb|S57971.1|S57971 Rh polypeptide II {clone RhPII... 36 3.4
        gi|36027|emb|X63094.1|HSRHII H.sapiens mRNA for rhesus poly... 36 3.4
        gi|18307511|emb|AJ428455.1|HSA428455 Homo sapiens partial R... 36 3.4
        gi|606991|gb|L37050.1|CHPRHC Pan troglodytes (clone 317-IIR... 36 3.4
        gi|1703665|gb|S82449.1|S82449 Homo sapiens D category IIIc ... 36 3.4
        gi|6759365|dbj|AB037270.1| Homo sapiens RhIVb(J) mRNA for R... 36 3.4
        gi|5360898|dbj|AB018969.1| Homo sapiens RhDYO mRNA for Rh b... 36 3.4
        gi|5360246|dbj|AB018968.1| Homo sapiens RhDVa(TT) mRNA for ... 36 3.4
        gi|5360244|dbj|AB018967.1| Homo sapiens RhDVa(TO) mRNA for ... 36 3.4
        gi|5360242|dbj|AB018966.1| Homo sapiens RhDVa(FK) mRNA for ... 36 3.4
        gi|12381901|dbj|AB046420.1| Homo sapiens RhDTI mRNA for Rh ... 36 3.4
        gi|337390|gb|L08429.1|HUMRHDANTI Human RhD blood group anti... 36 3.4

        Alignments


        >gi|28315973|tpe|BN000065.1|HSA000065 TPA: Homo sapiens SMP1 gene, RHD gene and RHCE gene
        Length = 315242

        Score = 46.1 bits (23), Expect = 0.004
        Identities = 26/27 (96%)
        Strand = Plus / Plus


        Query: 14 ctccggtccctgtccttgagttctgtg 40
        |||| ||||||||||||||||||||||
        Sbjct: 249813 ctccagtccctgtccttgagttctgtg 249839

        Score = 46.1 bits (23), Expect = 0.004
        Identities = 26/27 (96%)
        Strand = Plus / Minus


        Query: 14 ctccggtccctgtccttgagttctgtg 40
        |||| ||||||||||||||||||||||
        Sbjct: 165008 ctccagtccctgtccttgagttctgtg 164982

        Score = 36.2 bits (18), Expect = 3.4
        Identities = 18/18 (100%)
        Strand = Plus / Plus


        Query: 1 tggctgggctgatctccg 18
        ||||||||||||||||||
        Sbjct: 164689 tggctgggctgatctccg 164706

        >gi|49256907|emb|AL928711.18| Human DNA sequence from clone RP11-335G20 on chromosome 1 Contains the
        gene for small membrane protein 1 (SMP1), the RHD gene for
        Rhesus blood group D antigen, a succinate dehydrogenase
        complex subunit D integral membrane protein (SDHD)
        pseudogene and a CpG island, complete sequence
        Length = 105321

        Score = 46.1 bits (23), Expect = 0.004
        Identities = 26/27 (96%)
        Strand = Plus / Minus


        Query: 14 ctccggtccctgtccttgagttctgtg 40
        |||| ||||||||||||||||||||||
        Sbjct: 37744 ctccagtccctgtccttgagttctgtg 37718

        Score = 36.2 bits (18), Expect = 3.4
        Identities = 18/18 (100%)
        Strand = Plus / Plus


        Query: 1 tggctgggctgatctccg 18
        ||||||||||||||||||
        Sbjct: 37425 tggctgggctgatctccg 37442

        >gi|40733884|emb|BX855602.2| Human DNA sequence from clone XXyac-25BH1 on chromosome 1, complete
        sequence
        Length = 173910

        Score = 46.1 bits (23), Expect = 0.004
        Identities = 26/27 (96%)
        Strand = Plus / Minus


        Query: 14 ctccggtccctgtccttgagttctgtg 40
        |||| ||||||||||||||||||||||
        Sbjct: 27701 ctccagtccctgtccttgagttctgtg 27675

        这是BLAST连接:http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi
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