Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support

The loading of amplicons onto solid supports such as beads during multiplex PCR or emulsion PCR conventionally has been performed by use of Solid Phase PCR or asymmetric Solid Phase PCR. These approaches are restrictive with respect to amplification efficiency and degree of amplicon loading. This chapter details Enhanced Solid Phase PCR principles and methodologies to enable higher amplicon �loading in the context of uncompromised amplification efficiency.